The promyelocytic leukemia (PML) gene is a tumor suppressor gene associated with cell apoptosis, cell proliferation, and senescence. However, the role of PML in the ethanol-induced apoptosis is not fully-known. In this study, using wild-type mouse embryo fibroblasts (MEF) and PML null MEF cells, we found that (1) ethanol (100 mM and 200 mM) could obviously induce apoptosis of wild-type MEF cell...

1. Collect 5 ml of blood in a vacutainer tube (Becton Dickinson) containing EDTA and mix. 2. Make volume up to 10 ml with solution 1 (10 mM Tris pH 7.6; 10 mM KC1; 10 mM MgCy. 3. Add 120 /il Nonidet P40 (BDH) to lyse the cells. Mix well by inverting several times. 4. Spin down the nuclear pellet at 2000 rpm for 10 mins. 5. Pour off the supernatant without dislodging the pellet. The pellets can ...

1. Inhalation of vanadium compounds, particularly vanadate, is a cause of occupational bronchial asthma. We have now studied the action of vanadate on human isolated bronchus. Vanadate (0.1 microM-3 mM) produced concentration-dependent, well-sustained contraction. Its -logEC50 was 3.74 +/- 0.05 (mean +/- s.e.mean) and its maximal effect was equivalent to 97.5 +/- 4.2% of the response to acetylc...

P2 purinoceptor modulation of injury during ischemia-reperfusion was studied in murine hearts. Effects of P2 agonism or antagonism, and interstitial accumulation of P2 agonists (UTP, ATP, and ADP), were assessed in Langendorff perfused hearts during 20 min of ischemia and 45 min of reperfusion. In control hearts, ventricular pressure development recovered to 68 +/- 4 mm Hg (63 +/- 3% baseline),...

Exposure of wheat (Triticum durum L.) to NaCl (100 and 200 mM) showed that this variety is moderately halo tolerant. Germination rates in the absence and presence of salt (100 and 200 mM) are respectively 98.3, 83.3 and 53.3%. The supply of Salsola vermiculata and the combination between this halophyte and Streptomyces sp. SF5 affected germination significantly. Germination rates in the absence...

Analysis of DNA samples for restriction fragment length polymorphisms requires correct recognition of the normal restriction sites. Some restriction endonucleases have been shown to exhibit altered cut site specificity (star activity) under certain reaction conditions (1, 2). We report here the results of experiments demonstrating Hinfi star activity. Two ngs of pUC18 DNA or SV40 DNA were diges...

To quantify the interaction between epinephrine infusion and carotid sinus baroreceptor reflex control of vascular capacity and resistance, we have simultaneously measured total systemic compliance, Ct, arterial compliance, Ca, changes in "unstressed vascular volume," AV0, and resistance, R, in nine dogs whose carotid sinuses were isolated and cardiac output fixed by a perfusion pump. In respon...