Molecular tools have revolutionized the exploration of biodiversity, especially in organisms for which traditional taxonomy is difficult, such as for microscopic animals (meiofauna). Environmental (eDNA) metabarcode surveys of DNA extracted from sediment samples are increasingly popular for surveying biodiversity. Most eDNA surveys use the nuclear gene-encoding small-subunit rDNA gene (18S) as ...

Abstract Phylogenetic analyses of sequences the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS), and large (28S) region (= 18S-ITS-28S), as well this concatenated with largest RNA polymerase II ( RPB1 ) gene, proved that species originally described Acaulospora polonica (phylum Glomeromycota represents a new genus family ancient order Archaeosporales, here intro...

background: to differentiate sarcocystis macro-cyst-forming species in slaughtered sheep in babol area, mazandaran province, sequence analysis of 18s rrna gene was performed. methods: overall, 150 slaughtered sheep were examined macroscopically in slaughterhouse, babol and intra-abdominal and diaphragm muscles tissues infected with macro-cyst of sarcocystis spp. were collected in 2013. one macr...

cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. this study has aimed to investigate the hyper variable region of the 18s rrna gene in cryptosporidium for exact parasite identification . dna was extracted from 26 fecal samples from which initially cryptosporidium oocysts were identified by ziehl-neelsen acid-fast , auramine phen...

background: blastocystis is one of the most common parasites, reported from both human and animals. this parasite is more prevalent in regions with low levels of hygiene, close contact with animal and unsuitable disposal systems. the aim of the study was to subtype blastocystis sp., isolated from diarrheic and non-diarrheic patients using sequencing of 18s ribosomal dna. methods: totally, 300 s...