Conservation of genetic resources is essential for the continuation future crop production. Watermelon (Citrul-lus lanatus), a member Cucurbitaceae, widely distributed in tropical and subtropical regions. The aim this study was to reveal relationships with help microsatellite markers watermelon collection free unnecessary repetitions, determine success SSR (Simple Sequence Repeats) primers deve...

In this study, Pseudomonas syringe pathovars isolated from olive, tomato and bean were identified by species-specific PCR and their genetic diversity was assessed by repetitive extragenic palindromic (REP)-PCR. Reverse universal primers for REP-PCR were designed by using the bases of A, T, G or C at the positions of 1, 4 and 11 to identify additional polymorphism in the banding patterns. Bindin...

The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the g...

Safflower is a self-pollinating oilseed crop and has been cultivated since antiquity in the Middle East. become minor crop, to increase its cultivation it necessary characterize introduce new germplasm. Thirty-eight safflower cultivars breeding lines from different countries have screened with 8 AFLP primer combinations assess genetic diversity relationships among these genotypes. primers produ...

There is scanty information on tamarind’s genetic diversity in Easter Kenya. The objective of this study was to determine the 64 tamarind accessions from Eastern Kenya using 12 Inter Simple Sequence Repeat (ISSR) markers. DNA extracted young apical leaves modified CTAB method and amplified standard PCR. data collected were scored as presence (1) or absence (0) bands then compared 100bp ladder a...

The first generation of Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta) were subjected to species-specific PCR assays and the results showed that snails collected from the field were B. alexandrina, and there was no evidence for the presence of B. glabrata. The snails were subjected also to RAPD-PCR technique. The results showe...

In this study, 112 Echinococcus granulosus isolates from different livestock of Iran were genotyped by PCR amplification of ribosomal DNA-internal transcribed spacer 1 (rDNA-ITS1) region followed by restriction fragment length polymorphism (RFLP) with the enzyme RsaI. The possibility of intra-genotype variation was also investigated using randomly amplified polymorphic DNA (RAPD) analysis. Isol...

Powdery mildew caused by Erysiphe necator, is one of the most economically damaging diseases of grapevine throughout the world. This is the first molecular biology study on this fungus in Iran. The aims of the present study were (a) to analyse genetic diversity between isolates of E. necator fungus in four main grape production regions in Iran, i. e., Qazvin, Alamoot, Takestan and Shahriar and ...

The molecular variability of ten genotypes of Celosia argentea seeds collected from National Institute of Horticultural Research (NIHORT) and National Centre for Genetic Resources and Biotechnology (NACGRAB) germplasms were evaluated using the Amplified Fragment Length Polymorphism (AFLP) marker. The polymorphism of C. argentea was detected within the population using primer mix of AFLP EcoRI +...