In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been m...

Semen samples were collected from eight males of Goldfish (Carassius auratus gibelio) and cryopreserved using four extenders (Goldfish sperm extenders; GFSE 1– GFSE 4) supplemented with Methanol (MeOH) at concentration of 5%, 10% and 20%. Semen was diluted with ratio of 1:1 with extenders and frozen in liquid nitrogen vapor. Frozen sperms after 2 and 5 days were excluded from freezing. Experime...

This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with Androhep EnduraGuard (AeG), DILU-Cell (DC), SafeCell Plus (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17C. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive m...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

This study tested different concentrations of egg yolk in canine freezing extender void of glycerol, a commonly used cryoprotectant, by examining the motility and morphology throughout the freezing process: initial (baseline after extender added), post-cool (after three hours at 5°C) and post-thaw (after freezing.) Initial values of pH, osmolarity, motility and morphology were obtained for comp...

The influence of different extender osmolality levels and the presence of different cryoprotectants on the postthawed semen’s characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the cryoprotectants: 6% glycerol, 6% 1,2propanediol, 6...

The effects of different extenders and storage periods on the motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen were evaluated after short-term storage. Semen was collected from anesthetized males by the abdominal massage. After determination of main semen characteristics, the pooled ejaculates were diluted with 4 different extenders at a ratio of 1:3, and stored at 4...

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...