considering great medicinal value of hyssopus officinalis l. and possibility of its mass production through in vitro culture, two individual experiments was conducted. effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of tdz and bap in combination with 1 µmol of iaa on direct regeneration from nodal explants were assessed. significant difference between treatments was observed (p≤ 0.01...

Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

In vitro propagation of rose (Rosa indica) as well as rose breeding is a valuable economic enterprise and has received highest importance globally including Indian subcontinent. The development of new cultivars for commerce is faced with new challenges. In vitro micropropagation protocols of varieties of roses have been established but very few reports on the clonal propagation of local cultiva...

1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...

the effects of sucrose and some regulators on freezing tolerance in chickpea studied. four weeks in vitro explants were cultured in three different ms media without growth regulators and with normal level of sucrose (30 gl-1) (a), without growth regulators and with reduced sucrose (10 gl-1) (b) and medium supplemented with lmg gl-1 bap and 0.1 mgi1 iba and with 30 gi-1sucrose (c) for two weeks....

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration...

For efficient regeneration of Petunia × hybrida F1 'Opera Supreme Pink Morn', a simple in vitro micropropagation protocol was developed. Axillary bud explants dissected from 30-day-old in vitro-grown seedlings sprouted from hybrid seeds were cultured on Murashige and Skoog (MS) medium supplemented with 36 combinations of 6-benzylaminopurine (BA; 0.00, 0.50, 1.00, 2.00...