Rat liver cytosol contains two protein fractions capable of serving as benzo(a)pyrene (BP) carrier in in vitro oxidation of BP (0. Hanson-Painton, M. J. Griffin, and J. Tang, Biochem. Biophys. Res. Commun., 707: 1364-1371,1981). The role of the smaller carrier fraction in in vitro BP transport and oxidation was studied. Using Sephadex G-100 chromatography, [14C]BP bound to the carrier was shown...

The terahertz (THz) excitations in the quantum spin-ladder system Sr14Cu24O41 have been determined along the c axis using THz time-domain, Raman, and infrared spectroscopy. Low-frequency infrared and Raman active modes are observed above and below the charge-ordering temperature T(co) is approximately equal to 200 K over a narrow interval approximately equal to 1-2 meV approximately equal to 8-...

This paper presents the findings of a design, modeling, and fabrication study of ZnO and PbZr0 3Ti0 7O3 thin-film bulk acoustic resonators and filters. Measurements of the high-frequency responses of ZnO resonators having different area are used to develop an acoustic model that accurately represents resonator impedance data. The models are also used to interpret -parameter measurements on thin...

BACKGROUND AND OBJECTIVES DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length. Conserved sequences were selected for design of primers to generate DNA fragments of known specific size. MATERIALS AND METHODS In this study, we describe a method by which DNA lad...

background: positive attitude leads to a more successfully implementation of a change. we investigated the effect of attitudes of stakeholders toward a program on their prioritization of the program components for selecting the key question of a theory-driven evaluation with concept mapping method. materials and methods: during a brainstorming session, stated statements defined the program comp...

Solid-phase micro-reactors have been prepared in glass capillaries for DNA sequencing applications using slab gel electrophoresis, which consisted of a fused silica capillary (i.d. = 100 microns; o.d. = 365 microns; length = 15 cm; volume = 1.2 microL) that contained a covalently bound biotin molecule. With the addition of streptavidin to the capillary, an anchoring site was produced for the te...

BACKGROUND Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregating and identifying Leptospira serovars. The method has potential application in investigating the...

We report here that early apoptotic DNA fragmentation, as obtained by using an entirely new approach, is the result of an attack at a small number of specific open chromatin regions of interphase nuclei. This was demonstrated as follows: (i) chicken liver was excised and kept in sterile tubes for 1 to 3 hours at 37 degrees C; (ii) this induced apoptosis (possibly because of oxygen deprivation),...

We describe a modified method for typing a polymorphic microsatellite D12S391 locus by PCR using a newly designed primer pair. This primer pair produces shorter D12S391 amplified fragments (104-156 bp) than the primer pair originally described by Lareu et al. (209-261 bp). The detection system for the D12S391 locus using the new primer pair and capillary electrophoresis (CE) analysis was evalua...

The present study was undertaken to investigate the potential role of glucocorticoid-induced DNA damage in the lysis of human lymphoid leukemia cells by glucocorticoids. Lymphoblasts were isolated from patients with acute lymphoblastic leukemia (ALL) or chronic myelogenous leukemia (CML) in blast crisis and cultured in vitro with or without dexamethasone. DNA was then purified from the cells an...