نتایج جستجو برای: 10 protein
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The C. elegans genome contains sequences similar to a large number of mammalian genes implicated in the assembly, processing, and modification of glycans. In recent years, spectacular progress has been made in developing and refining tools to obtain structural information with small amounts of material, increasing our understanding of glycan structural complexity in this organism. These approac...
[This corrects the article DOI: 10.1371/journal.pone.0130940.].
Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a fu...
to determine the importance of q 10 h 2 as an antioxidant in cancer, we measured the activity of lipoamide dehydrogenase (q 10 h 2 recycling enzyme) in hl-60 and normal lymphocyte. the cultured cells of hl-60 and human normal lymphocytes were assayed in cell lysate of given number of both hl-60 and normal lymphocyte. the activity of lipoamide dehydrogenase and the protein concentration were det...
Destabilizing domains are conditionally unstable protein domains that can be fused to a protein of interest resulting in degradation of the fusion protein in the absence of stabilizing ligand. These engineered protein domains enable rapid, reversible and dose-dependent control of protein expression levels in cultured cells and in vivo. To broaden the scope of this technology, we have engineered...
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