This work established an integrated utilization of dairy whey in ?-galactosidase production from Lactobacillus bulgaricus and prebiotics synthesis by the probiotic enzyme. A cost-effective whey-based medium was newly developed for culturing to produce ?-galactosidase. The optimized through response surface methodology (RSM) involving a series statistical designs, such as Plackett–Burman design,...

Cancer cell drug resistance develops via several mechanisms, including apoptosis evading, efflux, reprogramming, DNA repair. Recently numerous data pointed out an ability of cancer cells reversibly exit from cycle to enter in G0 phase that may favor survival. Therefore, the goal study was determine transcriptional phenotype associated with quiescent/senescent state acquisition. Melanoma were tr...

The galactoside bond in cerebroside was found to be cleaved by an enzyme in rat and pig brain. Emulsified stearoyl-(14)C psychosine was used as the substrate and the extent of cleavage was studied by isolating and counting the stearoyl sphingosine (ceramide) formed. The reaction products, ceramide and galactose, were characterized by column and thin-layer chromatography. Cerebroside containing ...

D Galactose galactohydrolase (E.C.3.2.1.22), commonly referred to as galactosidase, catalyzes the hydrolysis of -galactosidic linkages in oligo-saccharide such as raffinose, melibiose, stachyose and verbascose, polysaccharides like galactomannans, and glycoconjugates such as glycoproteins and glycolipids. Ceramide trihexosides, its higher homologues and derivatives are also cleaved by the actio...

By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of producti...

BACKGROUND The study aim was to evaluate the immune reaction, difference of degenerative calcification, and anti-calcification effect of decellularization with or without α-galactosidase in bovine pericardium and porcine heart valves, using an α1,3-galactosyltransferase (α-Gal) knockout (KO) mouse model. METHODS In order to elucidate the anti-calcification effect of decellularization with or ...

b-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing b-D-galactosyl residues from b-D-galactosides. These enzymes have been detected in a wide range of plant organs and tissues. In a search for differentially expressed genes during the abscission process in citrus, sequences encoding b-galactosidase were identifi...

Galactosylceramide beta-galactosidase activity was assayed in cultured amniotic fluid cells from two pregnancies at risk for Krabbe's leucodystrophy. The elective termination of one pregnancy was carried out after demonstration of a severe deficiency of galactosylceramide beta-galactosidase activity. The diagnosis of Krabbe's leucodystrophy in the affected fetus was confirmed enzymatically by a...

The galactosidase immunosorbent test for carcinoembryonic antigen is simple to perform, uses stable reagents, does not require radioactive reagents, and is adaptable to large numbers of samples. Concentration of carcinoembryonic antigen in sera or plasma was determined by the galactosidase immunosorbent test and by the Egan-Todd double antibody assay (93% agreement), indirect Z-gel (83% agreeme...

A doença de Fabry é uma origem genética ligada ao cromossomo X. Ocorre devido a um erro inato no metabolismo dos glicoesfigolipídeos, o que provoca deficiência da enzima alfa-galactosidase A. O diagnóstico Doença na maioria das vezes desafiador. Desta forma, atuação do enfermeiro processo saúde paciente com indispensável educação e direcionamento tratamento adequado frente às complicações agrav...