Three commercially available kits that were supplemented with substrates for enzyme reactions were evaluated to determine their abilities to detect coliforms and fecal coliforms in foods. Japanese and U.S. Food and Drug Administration standard methods, as well as two agar plate methods, were compared with the three commercial kits. A total of 50 food samples from various retailers were examined...

Marine diatoms are known to be responsible for about a quarter of global primary production and their photosynthesis is sustained by inorganic carbon-concentrating mechanisms and/or C(4) metabolism. Activities of the inorganic carbon-concentrating mechanism are attenuated under enriched [CO(2)]; however, impacts of this factor on primary productivity and the molecular mechanisms of CO(2) respon...

A transgenic winter triticale line expressing the uidA gene, encoding ?-glucuronidase, was used to assess pollen flow in field experiments over two consecutive vegetation seasons central Poland. The experimental design included variants of mixed and non-transgenic lines. Pollen grains were collected using passive traps located at 0, 10, 30, 60 85 m from line. GM detected histochemically by stai...

Enterococcus faecalis and Uropathogenic Escherichia coli (UPEC) are the most common causal agents of urinary tract infections (UTI) infertility in humans. They secrete many cytolytic toxins that impact sperm functions spermatogenesis. The current paper aimed to investigate cytolysins among Bacteriospermia associated bacteria pyospermic patients. 110 Seminal fluid swabs were collected from patie...

هدف: از چالش­های عمده بشریت افزایش افسردگی و اختلال عملکرد جنسی ناشی داروهای ضدافسردگی است. با توجه به نقش سلول­های سرتولی در اسپرماتوژنز، پژوهش حاضر، اثر داروی دولوکستین را بر زنده­مانی، آپوپتوزیس بیان ژن­های Bax و­ (Connexin 43) Cx43 بررسی کرده مواد روش‌‌ها: TM4 محیط DMEM/F12 حاوی %5/2 FBS، 5% سرم اسب %1 پنی سیلین-استرپتومایسین کشت شدند. دوزهای 30،60، 15، 5/7، 75/3 میکرو­گرم/ میلی­لیتر زمان­ه...

background: sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. one of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. objectives: the objective of this research was to study the response of callus induc...

The development of genetically transformed plants is an elusive landmark in Cannabis sativa L. breeding. Despite its economic interest, at present, protocols for producing transgenic C. are scarce. We studied the ability hypocotyl, cotyledon and meristem explants from six hemp varieties plant regeneration. For this, we firstly evaluated vitro regeneration rates hypocotyls cultured medium withou...

We cloned and characterized the gene and cDNA of Aspergillus oryzae cytochrome P450nor (Anor). The Anor gene (nicA; CYP55A5) has a different gene structure from other P450nor genes in that it has an extra intron. There were not only two kinds of mRNA but also two sets of TATA-box and CCAAT-box, and it appears that this gene has two expression patterns, like CYP55A1 of Fusarium oxysporum. A repo...

Overall occurrence of Escherichia coli in the current study was 7.55% and corresponding group wise 13%, 2.66%, 10% 7% a faecal cloacal samples, environmental Retailer’s shop’s restaurant samples respectively. Amongst different type processed, highest recovery from collected at poultry farms (13%) followed by shop (10%) suggesting heavy infection contamination occurring initial points chain. In ...