We have developed a PCR-based assay which allows the detection of staphylococci at the genus level by targeting the tuf gene, which encodes the elongation factor Tu. Degenerate PCR primers derived from consensus regions of several tuf genes were used to amplify a target region of 884 bp from 11 representative staphylococcal species. Subsequently, the entire nucleotide sequence of these amplicon...

In order to improve the diagnosis of a Mycoplasma pneumoniae infection, we developed a polymerase chain reaction (PCR)-based assay. The gene encoding elongation factor Tu (tuf) was selected as the target sequence. Oligonucleotides derived from variable stretches of the tuf gene were able to prime the amplification of a 950-bp fragment exclusively when M. pneumoniae DNA was used as the template....

Biofilms help bacteria survive under adverse conditions, and the quorum sensing (QS) system plays an important role in regulating their activities. Quorum sensing inhibitors (QSIs) have great potential to inhibit pathogenic biofilm formation and are considered possible replacements for antibiotics; however, further investigation is required to understand the mechanisms of action of QSIs and to ...

وراثت مقاومت سفیدبالک گلخانه، Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae)، به دو ترکیب ایمیداکلوپرید و کلرپایریفوس مورد مطالعه قرار گرفت. در این پژوهش، از جمعیت فیلستان ورامین (FL) عنوان والد مقاوم فردیس کرج (FR) حساس استفاده شد. مقدار ترتیب حدودا 62/13 91/14 برابر بود. عدم وجود اختلاف معنی‌دار LC50 روی تلاقی‌های F1 (R♂×S♀) Fʹ1 (R♀×S♂) نشان داد که T. نوع اتوزومی (غیرجنسی) است. ه...

Abstract Twelve hyper-? carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing SSU rDNA phylogenetic analysis these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbc L tuf A their application study evaluated. Novel...

Batuan yang tersingkap di permukaan bumi umumnya dapat dimanfaatkan untuk kebutuhan konstruksi bangunan. Masyarakat Desa Candirejo, Kecamatan Semin, Kabupaten Gunungkidul, Daerah Istimewa Yogyakarta mengambil batu dengan cara sederhana yaitu penambangan tuf menggunakan pahat dan palu besi. Tujuan dari penelitian ini adalah menganalisis kestabilan lereng tambang akibat aktifitas oleh masyarakat ...