Many bacterial species exhibit a general stress response that can be induced by numerous very different stress conditions and, phenotypically, renders the cells broadly stress resistant. In Escherichia coli, this response is dependent on the sigmaS (RpoS) subunit of RNA polymerase. SigmaS is a close relative of the vegetative sigma factor sigma70 (RpoD) and recognizes very similar promoter sequ...

273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antib...

The sigma factor sigma 73 of the obligate intracytoplasmic bacterium Rickettsia prowazekii was overexpressed and purified from Escherichia coli. The rickettsial rpoD gene encoding sigma 73 was cloned into a Ndel-BamHI-cleaved pET-15b vector under control of T7 transcription and translation signals. The recombinant plasmid encoded a 75 kDa fusion protein that was overproduced in E. coli BL21(DE3...

The Pseudomonas chlororaphis phylogenetic subgroup of species, within the fluorescens group, currently includes seven bacterial all which have environmental relevance. Phylogenomic analyses help clarify taxonomy strains in group and allow for precise identification. Thirteen antibiotic-resistant isolated a previous study from nine different sampling sites Danube River were suspected to represen...

Housekeeping sigma factors in the σ family, as components of the RNA polymerase holoenzyme, are responsible for regulating transcription of genes related to vegetative growth. While these factors are well understood in model organisms such as Escherichia coli and Bacillus subtilis, little experimental work has focused on the sigma factors in members of the Lactobacillus genus such as Lactobacil...