An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the Kd is 6.9 microM, whereas for binding to ribosomes it is 0.7 microM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is in complexes with EF-G. Binding of EF-G to S/R RNA does not require GTP but is inhibited by ...

The GTPase-associated center in 23/28 S rRNA is one of the most conserved functional domains throughout all organisms. We detected a unique sequence of this domain in Bombyx mori species in which the bases at positions 1094 and 1098 (numbering from Escherichia coli 23 S rRNA) are C and G instead of the otherwise universally conserved bases U and A, respectively. These changes were also observed...

The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-3H into "32 S" RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-3 H incorporation into 32 S ...

Abstract Processes influencing the transport of airborne bacterial communities in atmosphere are poorly understood. Here, we report comprehensive and quantitative evidence key factors by dust plumes Eastern Mediterranean. We extracted DNA RNA from size-resolved aerosols sampled air masses different origins, followed qPCR high-throughput amplicon sequencing 16 S ribosomal gene transcripts. find ...

Abstract In eukaryotes three major nuclear RNA Polymerases (Pols I, II and III) transcribe the genome. Pols III many different genes. Pol I has only one target from which it synthesizes precursor for 3 of 4 ribosomal (r)RNAs accounting up to 60 percent total cellular RNA. Dedication its specific transcription factors a single gene underlines importance rRNA synthesis. Research in Regensburg aim...