AIM The present study was conducted to find the association among virulence determinants of verotoxic Escherichia coli (VTEC) isolated from cattle calf feces. MATERIALS AND METHODS A total of 216 cattle calf fecal samples were collected aseptically and processed under required conditions for the isolation of E. coli. The isolates were further subjected to multiplex polymerase chain reaction (...

conclusions based on our study, plca and hlya played a key role in the virulence determination of l. monocytogenes. data analysis also showed that l. monocytogenes could be a causative agent of abortion in pregnant women. results out of 96 samples, 16 isolates of l. monocytogenes by pcr (plca and hlya) and four isolates by culture were identified. there was a significant difference between pcr ...

We report a case of necrotizing fasciitis caused by Vibrio cholerae O137 in an immunocompromised 49-year-old man. The infection was acquired following a minor traumatic injury and exposure to seawater during the summer of 2009 in Italy. Although highly immunocompromised, the patient survived. The strain was cytotoxic, invasive, and adhesive and contained a fragment of the El Tor-like hemolysin ...

Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA, papG allele III, and iha. Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes (papG, the F7-2 papA allele, iutA, sat,...

Abstract Background Although Escherichia coli has been widely used for the expression of exogenous proteins, secretory in this system is still a big obstacle. As one most important secretion pathways, hemolysin A (HlyA) E. can transport substrates directly from cytoplasm to extracellular medium without formation any periplasmic intermediate, making it an ideal candidate development production p...

the aim of this study was to identify virulent shiga toxin-producing escherichia coli (stec) strains isolated from faecal samples of 100 clinically healthy calves. in the present study, a total of 100 escherichia coli (e. coli) isolates from clinically healthy calves belonging to 6 different farms located in khorasan razavi province, iran, were examined for presence of virulence genes character...

The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes...