Domain 5 (D5) is a highly conserved, largely helical substructure of group II introns that is essential for self-splicing. Only three of the 14 base pairs present in most D5 structures (A2.U33, G3.U32, and C4.G31) are nearly invariant. We have studied effects of point mutations of those six nucleotides on self-splicing and in vivo splicing of aI5 gamma, an intron of the COXI gene of Saccharomyc...

The diversity of Phytophthora spp. in rivers and riparian ecosystems has received considerable international attention, although little such research has been conducted in South Africa. This study determined the diversity of Phytophthora spp. within a single river in Gauteng province of South Africa. Samples were collected over 1 y including biweekly river baiting with Rhododendron indicum leav...

Mitochondrial aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in protein synthesis since they charge tRNAs with their cognate amino acids. Mutations in the genes encoding mitochondrial aaRSs have been associated with a wide spectrum of human mitochondrial diseases. Here we report the identification of pathogenic mutations (a partial genomic deletion and a highly conserved p. Asp325Tyr ...

ABSTRACT Polymerase chain reaction primers spanning the mitochondrially encoded coxI and II genes have been identified that were capable of amplifying target DNA from all 152 isolates of 31 species in the genus Phytophthora that were tested. Digestion of the amplicons with restriction enzymes generated species-specific restriction fragment length polymorphism banding profiles that were effectiv...

A new species of the genus Metaxonchium is described from natural habitats in Iran. The new species is characterized by having body length of 2.46-3.12 mm, lip region offset by constriction and 8-11 µm wide, odontostyle fusiform and 10-12 µm long, neck 773-1150 µm long, anterior portion of pharynx bearing a spindle-shaped thickening with valve-like structures inside, both parts of the pharynx s...

Abstract Background Aedes albopictus is an indigenous primary vector for dengue and Zika viruses in China. Compared with its insecticide resistance, biology competence, little known about genetic variation, which corresponds to environmental variations. Thus, the present study examines how Ae. varies among different climatic regions China deciphers potential dispersal patterns. Methods The vari...

Abstract The East European vole Microtus mystacinus is the most widespread species in Anatolia. It also frequently seen watery habitats a large area of Eurasia. In this paper, an attempt was made to ascertain level genetic differentiation between Anatolian (Asian part Turkey) and Turkish Thracian (European populations together with additional data from other parts Asia Europe by analysing two m...