Transport if loaded uclear transporters can be viewed as taxis that move cargo across the nuclear envelope. On page 649, Plafker et al. report that importin-11, a nuclear transport receptor , may be an especially selective cabbie. Importin-11 transports UbcM2, a ubiquitin (Ub)-conjugating enzyme, but it does so only when the enzyme is charged with a Ub at its active site. UbcM2 is an E2 enzyme,...

Transport if loaded uclear transporters can be viewed as taxis that move cargo across the nuclear envelope. On page 649, Plafker et al. report that importin-11, a nuclear transport receptor , may be an especially selective cabbie. Importin-11 transports UbcM2, a ubiquitin (Ub)-conjugating enzyme, but it does so only when the enzyme is charged with a Ub at its active site. UbcM2 is an E2 enzyme,...

export by binding directly to the exportin, Crm1, forming a complex that has a higher affinity for RanGTP and cargo than has Crm1 alone (Englmeier et al., 2001; Lind-say et al., 2001). Crm1:RanBP3:RanGTP:cargo exports Summary from the nucleus to the cytoplasm and is disassembled by RanBP1 and the Ran GTPase activating protein, Ran-Many nuclear-targeted proteins are transported through the nucle...

Transport if loaded uclear transporters can be viewed as taxis that move cargo across the nuclear envelope. On page 649, Plafker et al. report that importin-11, a nuclear transport receptor , may be an especially selective cabbie. Importin-11 transports UbcM2, a ubiquitin (Ub)-conjugating enzyme, but it does so only when the enzyme is charged with a Ub at its active site. UbcM2 is an E2 enzyme,...

granules in spinal cord axons regenerating into an ascending peripheral nerve graft in a rendered xyz scan. The green channel shows axons expressing neurofilament and SCG10, the orange channel shows Importin 1 mRNA in axons, the red channel shows non-neuronal Importin 1 mRNA, and the blue channel shows Schwann cell nuclei. For more information, see the article by Kalinski et al. (pages 10357–10...

We have previously reported that mutations in the polymerase proteins PB1, PB2, PA, and the nucleocapsid protein NP resulting in enhanced transcription and replication activities in mammalian cells are responsible for the conversion of the avian influenza virus SC35 (H7N7) into the mouse-adapted variant SC35M. We show now that adaptive mutations D701N in PB2 and N319K in NP enhance binding of t...

Spindle assembly requires the coordinated action of multiple cellular structures to nucleate and organize microtubules in a precise spatiotemporal manner. Among them, the contributions of centrosomes, chromosomes, and microtubules have been well studied, yet the involvement of membrane-bound organelles remains largely elusive. Here, we provide mechanistic evidence for a membrane-based, Golgi-de...

Antiestrogens including tamoxifen and fulvestrant have been evaluated as chemotherapeutics for ovarian cancer, particularly in cases of platinum resistant disease. Human epididymis protein 4 (HE4) is highly overexpressed in women with ovarian cancer and overexpression of HE4 has been found to correlate with platinum resistance. However, the role of HE4 in modulating responses to hormones and ho...