Seventeen rat GABAA receptor subtypes were transiently expressed in the human embryonic kidney 293 cell line from alpha 1, alpha 2, alpha 3, alpha 5, or alpha 6 variants with any of the three beta subunits and gamma 2S or gamma 3. We obtained fingerprints in the form of subtype characteristic concentration-response curves of 35S-TBPS binding to GABA and the GABAA antagonists SR 95531 and bicucu...

A capsular polysaccharide from Escherichia coli K5 was previously found to have the same structure, [-(4)beta GlcA(1)----(4)alpha GlcNAc(1)-]n, as that of the non-sulphated precursor polysaccharide in heparin biosynthesis [Vann, Schmidt, Jann & Jann (1981) Eur. J. Biochem. 116, 359-364]. The K5 polysaccharide was N-deacetylated (by hydrazinolysis) and N-sulphated, and was then incubated with de...

t-Butylbicyclophosphorothionate (TBPS) is a bicyclophosphate derivative with potent picrotoxin-like convulsant activity that binds with high affinity and specificity to a Cl- channel-modulatory site of the gamma-aminobutyric acid (GABA)/benzodiazepine receptor complex. Using intact cerebellar granule cells maintained in primary culture, we have studied the modifications induced by GABA and diaz...

Tobacco plants were transformed with a Nhap type Na /H antiporter gene, SynnhaP1 (slr1595), from a cyanobacterium Synechocystis sp. PCC 6803. Two kinds of promoters, Arabidopsis alcohol dehydrogenase gene promoter (Adh promoter) and CaMV 35S promoter (35S promoter), were used. The transgenic plants driven by Adh promoter accumulated SynNhaP1 proteins only in root whereas the transgenic plants d...

Chlamydomonas reinhardii CW 15, Higher Plant Lipids, Diacylsulphoquinovosyl Glyceride, Sulphonyl Group Incorporation, Adenosine 3'-phosphate 5'-Sulphatophosphate Biosynthesis of the plant sulpholipid (diacylsulphoquinovosyl glycerol) has been demonstrated in a cell-free assay system from the green alga Chlamydomonas reinhardii CW 15. The sulpholipid was labelled with [35S]sulphate when ATP, Mg2...

A previous communication has reported the specific uptake of 35S sulphate by the myeloid cells of human bone marrows in vitro (Lajtha, Ellis, and Oliver, 1953). Experiments in vivo on rats and rabbits confirmed the findings in vitro: uptake by the early myeloid cells, and no uptake by the nucleated red cells or lymphocytes. Repeated large doses of 35S sulphate (1-2,uC/g. daily for seven days) w...

Wilcox, P., and Patrick, A. D. (1974). Archives of Disease in Childhood, 49,209. Biochemical diagnosis of cystinosis using cultured ceils. A simple method is described for the biochemical diagnosis of cystinosis using small numbers of cells cultured in medium containing L-35S-cystine. Nonprotein sulphur-labelled compounds were extracted from the cells and separated by thin-layer chromatography....

Activation of kappa receptors inhibits adenylate cyclase, enhances K+ conductance and reduces Ca++ conductance via pertussis toxin-sensitive G proteins. We recently cloned a human kappa opioid receptor and stably expressed it in Chinese hamster ovary (CHO) cells. In this study, the effects of activation of the human kappa receptor by agonists on [35S]GTPgammaS binding to CHO cell membranes were...

A C6 glioma cell line stably transfected with the human kappa opioid receptor (kappaOR) was used to characterize receptor binding and G protein activation via the kappaOR by a comprehensive series of opioid ligands. The ligand-binding affinity for [3H]5alpha,7alpha, 8beta(-)-N-methyl-N-(7-Cl-pyrrolidinyl)-1-oxaspiro(4, 5)dec-8-yl)benzene acetamide (U69593) was similar to that observed in monkey...