The hepatitis C virus (HCV) envelope proteins, E1 and E2, form noncovalent heterodimers and are leading candidate antigens for a vaccine against HCV. Studies in mammalian cell expression systems have focused primarily on E2 and its folding, whereas knowledge of E1 folding remains fragmentary. We used a cell-free in vitro translation system to study E1 folding and asked whether the flanking prot...

the object of this cross sectional study was to determine the hcv subtype 3a envelope protein binding affinity with human leukocyte antigen. envelope 1 (e1) protein is one of the structural proteins responsible for entering the cells through the receptors. the binding affinity of e1 protein epitopes to the selected human leukocyte antigen (hla) class i alleles was investigated using the compute...

We have generated replication-competent (VSV-C/E1/E2) and nonpropagating (VSVDeltaG-C/E1/E2) vesicular stomatitis virus (VSV) contiguously expressing the structural proteins of hepatitis C virus (HCV; core [C] and glycoproteins E1 and E2) and report on their immunogenicity in murine models. VSV-C/E1/E2 and VSVDeltaG-C/E1/E2 expressed high levels of HCV C, E1, and E2, which were authentically po...

BACKGROUND The aim of this study was to investigate whether the anisotropy parameters are helpful in the detection and discrimination of breast cancers, and to determine its value in predicting the risk of cancers. MATERIAL AND METHODS There were 56 patients with 56 lesions (34 malignant, 22 benign) included in the study. DTI was performed in every patient and apparent diffusion coefficient (AD...

Replication of bovine papillomavirus type 1 DNA absolutely requires the viral transcription factor E2 as well as the initiator E1, although E1 alone has all the activities expected of an initiator protein. E1 assembles on the DNA in a stepwise fashion and undergoes a transition in activities from site-specific DNA-binding protein to mobile helicase. Complex assembly is assisted by the viral tra...

and Applied Analysis 3 Conversely, if the pair of functions α and β satisfy the conditions 1 – 4 , then there exists a unique u ∈ E1 such that u λ α− λ , β λ for each λ ∈ 0, 1 , 9 . The fuzzy number u corresponding to the pair of functions α and β is defined by u : R → 0, 1 , u x sup{λ : α x ≤ λ ≤ β x }, 5 . A sequence u uk of fuzzy numbers is a function u from the set N, the set of all positiv...

The spikes of alphaviruses are composed of three copies of an E2-E1 heterodimer. The E1 protein possesses membrane fusion activity, and the E2 protein, or its precursor form, p62 (sometimes called PE2), controls this function. Both proteins are, together with the viral capsid protein, translated from a common C-p62-E1 coding unit. In an earlier study, we showed that the p62 protein of Semliki F...