We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP-F 14-1...

The biology and genetics of silkworm, Bombyx mori, is the most advanced of any lepidopteran species. Its rich repertoire of genetic resources and potential applications in sericulture and as a model for other Lepidoptera led to the initiation of genomics research. During the past decade much effort has been made in the areas of marker development, and molecular maps have been constructed in sta...

Miniand microsatellite 'fingerprint' probes have provided animal geneticists and behavioural ecologists with a powerful means of exploring fine scale differentiation and breeding behaviour in natural populations (1, 2). Unfortunately, the development of fingerprint technology in plants has been disappointingly slow. There are relatively few reports of the use of minisatellite probes in plants (...

The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 ...

The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification produced by bot...

Red or variegated chicory (Cichorium intybus L., 2n=2x=18) native to, and very extensively cultivated in North-Eastern Italy as a leafy vegetable, locally called “radicchio”, includes different types which represent valuable high-quality crops. The five major types of radicchio cultivated in the Veneto region were investigated by PCR-derived molecular markers (e.g. RAPDs, AP-PCRs, I-SSRs and AF...

Random amplified polymorphic DNA (RAPD) markers can be generated using short arbitrary primers to amplify genomic DNA, giving a genotype-specific pattern of bands. RAPD analysis should lead to the saturation of the genome without the requirement of previous genetic information and using few expensive oligonucleiotides. The use of DNA marker technology in poultry as a strains identification has ...

Molecular and morphometric analyses of different Indian and Sri Lankan populations of two rattan species (Calamus rivalis and C. metzianus) were carried out using Random Amplified Polymorphic DNA (RAPD) markers and morphological characters. Multivariate analysis of RAPD and morphological data failed to separate these populations into two distinct species. RAPDs generated a total of 117 markers ...