Nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) may repress the interferon (IFN)-induced protein kinase R (PKR). High variability of different regions in the carboxy-terminal half of NS5A implicated in the interaction with PKR (particularly the interferon sensitivity determining region (ISDR)) may be a predictor of response to IFN in patients infected with genotype 1b of HCV. We ...

By the methods of bioinformatics, the sequences of HCV E2 protein from all the genotypes are studied on the variation and the B-cell epitopes. We find that even in the HVR1 and HVR2 regions, there are also some conservative sites, such as T2, G6, G23, and Q26, all of which belong to polar R-base amino acids without charge, and can lost proton to conform hydrogen bond. Meanwhile, we find the con...

In a prospective study of 33 infants born to hepatitis C virus (HCV)-positive human immunodeficiency virus-negative mothers the vertical transmission of HCV occurred in 6.8%. The evolution of HCV infection in two babies was studied from birth up to 5 or 6 years of age, and the sequencing of the hypervariable region (HVR) of the putative envelope-encoding E2 region of the HCV genome was performe...

BACKGROUND Injecting drug users remain the population at greatest risk of acquiring hepatitis C virus (HCV) infection, although a recent increase in cases of sexually transmitted HCV infection has been observed among human immunodeficiency virus (HIV)-infected individuals. The extent to which these separate epidemics overlap is unknown. METHODS The Australian Trial in Acute Hepatitis C (ATAHC...

در سال‌های أخیر با افزایش پیچیدگی، سطح عدم­اطمینان نیز یافته است؛ ازاین‌رو مدیریت ریسک زنجیره تأمین یکی از موضوع‌هایی است که موردتوجه سازمان‌ها قرار گرفته است. ریسک‌های موجود تأمین، وارده ناحیه تأمین‌کنندگان پژوهش حاضر به‌کارگیری مدلی به‌منظور ارزیابی صنایع غذایی شی­‌فو، به شناسایی محتمل و اتخاذ تصمیماتی راستای می­‌پردازد. مدل به‌کار‌گرفته‌شده، ترکیبی محاسبات کلامی فازی (FLC) محاسبه مقدار عوامل...

به منظور ساخت، راه‌اندازی و عیب‌یابی مقدماتی چشمه پنینگ داخلی سیکلوترون 10IRANCYC، یک مگنت الکتریکی دوقطبی با میدان مغناطیسی در حد امکان مشابه مرکز مذکور طراحی ساخته شد. این الکترومگنت که دانشکده فیزیک دانشگاه تهران شد، قادر است بزرگی 7000 گوس را محل استقرار (منطقه‌­ای شعاع 15 میلی‌متر مگنت) آسانی بدون نیاز خنک‌سازی تأمین کند. دست‌­یابی 1/1 تسلا نیز استفاده از برای مدت چند دقیقه امکان‌­پذیر است...

Globally, hepatitis C Virus (HCV) infection is responsible for a large proportion of persons with liver disease, including cancer. The infection is highly prevalent in sub-Saharan Africa. West Africa was identified as a geographic origin of two HCV genotypes. However, little is known about the genetic composition of HCV populations in many countries of the region. Using conventional and next-ge...

HIV/HCV coinfected individuals under highly active antiretroviral therapy (HAART) represent an interesting model for the investigation of the role played by the immune system in driving the evolution of the HCV quasispecies. We prospectively studied the intra-host evolution of the HCV heterogeneity in 8 coinfected subjects, selected from a cohort of 32 patients initiating HAART: 5 immunological...

Reports of cerebral dysfunction in chronic hepatitis C virus (HCV) infection have led to the suggestion that HCV may infect the central nervous system (CNS). We used reverse transcription-PCR, cloning, and sequencing to define quasispecies for the HCV internal ribosomal entry site (IRES) and hypervariable region 1 (HVR1) in autopsy-derived brain, liver, lymph node, and serum samples. There was ...

Sequencing is important for understanding the molecular epidemiology and viral evolution of hepatitis C virus (HCV) infection. To date, there is little standardisation among sequencing protocols, in-part due to the high genetic diversity that is observed within HCV. This study aimed to develop a novel, practical sequencing protocol that covered both conserved and variable regions of the viral g...