A detailed characterization of RuvB protein-mediated ATP hydrolysis in the presence of RuvA protein has provided (a) the steady-state kinetic parameters of ATP hydrolysis within a RuvAB complex and (b) several insights into the mechanism of ATP hydrolysis and its coupling to translocation on DNA. In general, the RuvA protein increases the kcat and decreases the Km for the RuvB ATPase activity. ...

The systemic effects of physical activity are mediated by the release IL-6 and other myokines from contracting muscle. Although muscle has been extensively studied, information on cellular mechanisms is fragmentary scarce, especially regarding role Ca2+ signals. aim this study was to characterize main components signals in human skeletal cells during secretion stimulated mobilizing agonist ATP....

Hydrolysis of ATP by partition ATPases, although considered a key step in the segregation mechanism that assures stable inheritance of plasmids, is intrinsically very weak. The cognate centromere-binding protein (CBP), together with DNA, stimulates the ATPase to hydrolyse ATP and to undertake the relocation that incites plasmid movement, apparently confirming the need for hydrolysis in partitio...

Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM...

A heterogeneous particle catalyst (CuO-Fe2O3/CTS-ATP) was synthesized via injection molding and ultrasonic immersion method, which is fast effective. The applied attapulgite (ATP) wrapped by chitosan (CTS) as support, loaded dual metal oxides CuO Fe2O3 active components. After a series of characterizations catalysts, it found that were successfully evenly on the surface CTS-ATP support. used to...

Tujuan dari penelitian ini adalah menganalisis nilai kemampuan membayar (Ability To Pay/ ATP) dan kesedian (Willingness Pay/WTP) masyarakat dalam hal pengguna untuk jasa layanan angkutan umum, dengan menggunakan contingent valuation method. Dan mengidentifikasi variable yang berpengaruh terhadap ATP, WTP Pendapatan penyedia ordinary least square Hasil menunjukkan atau tingkat kemauan rata-rata ...

Arginyl dipeptides like Arg-Ser, Arg-Ala, and Arg-Gly are salt-taste enhancers can potentially be used to reduce the salt content of food. The l-amino acid ligase RizA from B. subtilis selectively synthesizes arginyl dipeptides. However, industrial application is prevented by high cost cofactor adenosine triphosphate (ATP). Thus, a coupled reaction system was created consisting acetate kinase (...

A label-free fluorescence aptasensor was studied for adenosine triphosphate (ATP) detection that combines low biosensor toxicity with a simplified preparation process. In this study, the aptamer of gold nanoparticle@aptamer@carbon quantum dot nanostructure could specifically identify ATP, resulting in change signal. order to analyze performance sensor, effects carbon (CQD) concentration and cen...

ATP hydrolyzing activity of a mutant alpha3beta3gamma subcomplex of F0F1-ATP synthase (DeltaNC) from the thermophilic Bacillus PS3, which lacked noncatalytic nucleotide binding sites, was inactivated completely soon after starting the reaction (Matsui, T., Muneyuki, E. , Honda, M., Allison, W. S., Dou, C., and Yoshida, M. (1997) J. Biol. Chem. 272, 8215-8221). This inactivation is caused by rap...