Replication in vitro of the replicative form (RF) I DNA of bacteriophage varphiX174 requires the phage-induced cistron A (cisA) protein, the host rep protein, DNA-binding protein, ATP, and DNA polymerase III plus replication factors. The rep protein is a single-stranded DNA-dependent ATPase. In this paper we show that varphiX174 RF I DNA cut by the cisA protein acts as a duplex DNA cofactor for...

A typical B-rep describes a solid in its full detail. Operations that locally analyze the B-rep usually process the entire B-rep due to the lack of spatial ordering of its topological entities. For very complex objects, this global processing is time consuming and yet unnecessary. One approach in avoiding the global processing is to use several B-reps of different levels of detail. However, thi...

The current study was conducted to determine the usefulness of 2 molecular techniques, automated repetitive extragenic palindromic-PCR (REP-PCR) and denaturing gradient gel electrophoresis (DGGE), to identify Salmonella serotypes of poultry origin. Salmonella continues to be a foodborne pathogen of principal concern in the United States. The interspersed conserved repetitive sequence of the bac...

Repetitive extragenic palindrome (REP)-associated tyrosine transposase enzymes (RAYTs) bind REP DNA domains and catalyze their cleavage. Genomic sequence analyses identify potential noncoding REP sequences associated with RAYT-encoding genes. To probe the conformational space of potential RAYT DNA binding domains, we report here spectroscopic and calorimetric measurements that detect and partia...

High mobility group protein 1 (HMG1) is an abundant non-histone chromosomal protein which plays a role in several nuclear events involving DNA. Here we demonstrate that HMG1 physically interacts with the human adeno-associated virus (AAV) Rep protein. HMG1 promotes the formation of Rep-DNA complexes and stimulates the activity of Rep in site- and strand-specific cleavage of DNA and the hydrolys...

Adeno-associated virus type 2 (AAV) is known to establish latency by preferential integration in human chromosome 19q13.42. The AAV non-structural protein Rep appears to target a site called AAVS1 by simultaneously binding to Rep-binding sites (RBS) present on the AAV genome and within AAVS1. In the absence of Rep, as is the case with AAV vectors, chromosomal integration is rare and random. For...

in this research, the effect of micro turbulence on the flotation rate of quartz particles was investigated. the maximum particle reynolds number (rep) was obtained at 60.25 with a particle size of -500+420 µm, impeller speed of 900 rpm, bubble surface area flux of 10.21 1/s and micro scale turbulence size of 162 µm. when the micro turbulence size was equal to the particle size, the maximum flo...

nutritive value of rumen epithelial scraping (rep) was assessed using in sacco and in vivo procedures. concentrates were so formulated that 0% (a), 35% (b), 70% (c), and 100% (d) of soybean meal (sbm), were replaced (weight for weight) with rep. in a completely randomized design, 16 west african dwarf (wad) goats were randomly allocated to the experimental diets to assess feed intake and digest...

OBJECTIVE The purpose of this study was to evaluate the effectiveness of the use of Reference Ear Plug (REP) during cone-beam computed tomography (CBCT) scan for the generation of lateral cephalograms from CBCT scan data. METHODS Two CBCT scans were obtained from 33 adults. One CBCT scan was acquired using conventional methods, and the other scan was acquired with the use of REP. Virtual late...