Interleukin-12 (IL-12) has been used in numerous immunotherapy protocols against melanoma. However, delivery of IL-12 in the form of recombinant protein can result in severe toxicity, and gene therapy has had limited success against B16.F10 murine melanoma. The purpose of this study was to examine the effectiveness of in vivo electroporation for the delivery of plasmid DNA encoding IL-12 as an ...

Pseudomonas sp. MX-058 produces aldehyde oxidase catalysing glyoxal to glyoxylic acid. Two aldehyde oxidases (F10 and F13) were purified to homogeneity from Pseudomonas sp. MX-058. F10 and F13 had subunit structures, a heterotetramer and heteropentamer respectively. The N-terminal amino acid sequences of all subunits were highly homologous to amino acid sequences of the putative oxidoreductases...

This study was conducted to compare the physicochemical traits of dry-cured hams made from two different three-way crossbred pigs: Yorkshire×Landrace×Duroc (YLD) and Yorkshire×Berkshire×Duroc (YBD). Animals were slaughtered at a live weight of 110 to 120 kg and cooled at 0°C for 24 h in a chilling room, the ham portion of the carcasses were cut and processed by dry-curing for physico-chemical a...

The objective of this study was to investigate the effects of mixed starter cultures isolated from kimchi on physicochemical properties, functionality and flavors of fermented ham. Physicochemical properties, microbial counts, shear force, cholesterol contents and volatile compounds of fermented ham were investigated during processing (curing and ripening time). Curing process for 7 d increased...

Qualitative and quantitative 'analyses of cell surface Sialylglycoproteins and glycosphingolipids of in vivo se lected mouse melanoma variant lines that show either a high (F10) or low (F1) degree of lung implantation have been made in culture and in in wVo-grown tumors. The following observations have been made concerning the cell surface characteristics of the two cell lines: (a) al though th...

Abstract Wave energy converters (WECs) are built to extract wave energy. However, this kind of device is still expensive for commercial utilization. To cut down the cost WECs by sharing construction with breakwaters, an integrated cylindrical WEC-type breakwater system that includes a WEC array in front very long proposed and attenuate incident waves. This paper aims optimize performance system...

Starch is a biodegradable polymeric carbohydrate that can easily form films and coatings readily be obtained from some food industry by-products wastes, which may contribute to the circular bioeconomy. In this work, we studied potential of two edible coating emulsions based on pregelatinized potato starch (PPS) glyceryl monostearate (GMS) alone (F6 F10) or formulated with additive sodium benzoa...

Considering the fact that iRGD (tumor-homing peptide) demonstrates tumor-targeting and tumor-penetrating activity, and that B16-F10 (murine melanoma) cells overexpress both αv integrin receptor and neuropilin-1 (NRP-1), the purpose of this study was to prepare a novel doxorubicin (DOX)-loaded, iRGD-modified, sterically-stabilized liposome (SSL) (iRGD-SSL-DOX) in order to evaluate its antitumor ...

Spanish dry-cured ham is a high quality product whose economic value is mainly given by its curing time. An intense proteolysis takes place throughout the dry-cured processing, which results in the generation of a high amount of peptides and free amino acids responsible for the final quality of dry-cured hams. In this work, a peptidomics approach has been used to study the evolution of peptides...

Market pigs (n = 120) were rested 0, 1, 2, or 3 h before slaughter. One carcass side was hot-fat trimmed (HFT) immediately after dressing, and the other was not fat trimmed (NFT). Sides received conventional chilling (CC) or accelerated chilling (AC) in a freezer at -32 degrees C for 100 min. Skin temperatures of live pigs decreased 5 degrees C during 3 h of rest, and resting reduced muscle tem...