When purified antihemophilic factor (Factor VIII) was rechromatographed on 4% agarose in 0.15 M NaCl or 1.0 M NaCl, a single protein peak, containing both procoagulant activity and von Willebrand factor activity, as defined by ristocetin-induced platelet aggregation, was eluted in the void volume. Purified Factor VIII immediately lost about 30% of its procoagulant activity when dissolved in 0.2...

Gavage of 2 M NaCl (IG 2 M NaCl), a procedure to induce cell-dehydration-and water and 0.15 M NaCl intake in a two-bottle choice test-is also a potential gastric irritant. In this study, we assessed whether mineral intake induced by IG 2 M NaCl is associated with gastric irritation or production of pica in the rat. We first determined the amount of mineral solution (0.15 M NaCl, 0.15 M NaHCO3, ...

Using response surface methodology (RSM), this study investigates the effect of NaCl substitution (50%) with KCl, CaCl2, and MgCl2 in packaging brines (controlled variables) on characteristics (responses) plain green Spanish-style Manzanilla olives, maintaining salt-mixture level 5%. The RSM showed that increment CaCl2 caused a linear significant (p-value ≤ 0.05) decrease pH increase firmness (...

Four diluents were compared as reference standards for the assay of gentamicin in cerebrospinal fluid (CSF): human CSF, human serum, distilled water, and 150 mmol NaCl/4.5 mmol CaCl2. Standards prepared in pooled human serum were the best alternative to CSF for the assay of gentamicin and were also useful for the assay of tobramycin, netilmicin, amikacin, and sisomicin. The pH (6.0-9.8) of CSF ...

Fusion of multilamellar phospholipid vesicles with planar phospholipid bilayer membranes was monitored by the rate of appearance in the planar membrane of an intrinsic membrane protein present in the vesicle membranes. An essential requirement for fusion is an osmotic gradient across the planar membrane, with the cis side (the side containing the vesicles) hyperosmotic to the opposite (trans) s...

potassium (k) is an element essential for plant growth, playing a significant role in physiological and biochemical processes in plant. the relationship between a soil testing extractant vs. plant nutrient uptake should be a determining parameter in the selection of a soil testing method. the objective of this study was to evaluate different extractants employed for extracting available k in 10...

Various electrolytes in test solutions applied to the skin were evaluated with regard to their effects on enhanced skin permeation of calcein as a model permeant by electroporation (EP), which is a physical means to increase skin penetration by applying a high voltage pulse on the skin surface. Calcein solution (1.0 mM) containing different electrolytes at a concentration of 150 mM was applied ...

High-resolution atomic force microscopy is used to map the surface charge on the basal planes of kaolinite nanoparticles in an ambient solution of variable pH and NaCl or CaCl2 concentration. Using DLVO theory with charge regulation, we determine from the measured force-distance curves the surface charge distribution on both the silica-like and the gibbsite-like basal plane of the kaolinite par...