In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD is a modification of the PCR in which a single, short and arbitrary oligonucleotide primer, able to anneal and prime at multiple locations throughout the genome, can produce a spectrum of a...

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the cons...

OBJECTIVE To evaluate genus- and species-specific polymerase chain reactions (PCRs) for the detection of the genus Legionella and the species Legionella pneumophila in clinical specimens and hospital water supplies, and to establish a simple and reproducible random amplification of polymorphic DNA (RAPD)-PCR technique for genotyping of Legionella. MATERIALS AND METHODS A total of 70 respirato...

conclusions our results showed that blaoxa-23 was the most prevalent gene, followed by blaoxa-51, among the burn and non-burn clinical isolates of a. baumannii. blaoxa-24-like genes were detected at a lower level and were only found among the burn isolates, which also showed higher heterogeneity compared to the non-burn group. background emergence of multidrug-resistant acinetobacter baumannii ...

The purpose of this study was to determine the utility of RAPD-PCR and antibiotic susceptibility tests in differentiation of S. typhimurium isolates in Iran. Thirty isolates of S. typhimurium, selected based on animal source; place and time of the isolation and a reference strain of the bacterium were used in this study. Serotyping of the isolates was performed by reliable antisera and confirme...

Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (P...

Introduction The quality of DNA, obtained by various protocols and subjected to various storage conditions can be judged by comparing the PCR amplification profiles. The most commonly used technique that can be accessed by almost any laboratory is RAPD . Random amplified polymorphic DNA (RAPD) is a PCR based technique for identifying genetic variation. It involves the use of a single arbitrary ...