Mouse ovarian aryl hydrocarbon hydroxylase (AHH, EC 1.14.14.2) activity was measured in control mice and in DBA/ 2N (hereafter called D2) and C57BL/6N (hereafter called B6) mice treated with 3-methylcholanthrene (MC). Basal ovarian AHH activity was similar in both strains (3 pmol/mg/min). Ovarian AHH was induced 2to 3-fold in B6 mice after MC treatment, while no change was observed in similarly...

OBJECTIVE Higher latitude, lower ultraviolet exposure, and lower serum 25-hydroxyvitamin D (25OHD) correlate with higher multiple sclerosis (MS) prevalence, relapse rate, and mortality. We therefore evaluated the effects of high-dose vitamin D2 (D2) in MS. METHODS Adults with clinically active relapsing-remitting MS (RRMS) were randomized to 6 months' double-blind placebo-controlled high-dose...

The C3H/HeJ mouse presents an inherited type 1 deiodinase (D1) deficiency that results in elevated serum thyroxine (T(4)), whereas TSH and tri-iodothyronine (T(3)) concentrations are normal when compared with those in the C57BL/6J strain. Here, we evaluated the expression of the type 2 (D2), the other T(4)-activating enzyme, in C3H mice. A comparative analysis revealed that D2 mRNA levels in C3...

The distribution of mRNA coding for the D2 dopamine receptor was studied in the rat brain by in situ hybridization. A cDNA probe corresponding to the putative third cytosolic loop and sixth and seventh transmembrane domains of the rat D2 receptor was used to generate an 35S-labeled riboprobe to hybridize to D2 receptor mRNA. D2 mRNA was found both in dopamine projection fields and in regions as...

Let I be the ideal generated by alternating polynomials in two sets of n variables. Haiman proved that the q, t-Catalan number is the Hilbert series of the graded vector space M(= ⊕ d1,d2 Md1,d2) spanned by a minimal set of generators for I. In this paper we give simple upper bounds on dim Md1,d2 in terms of partition numbers, and find all bi-degrees (d1, d2) such that dimMd1,d2 achieve the upp...

The non-catechol, selective dopamine D2-agonist compound 3H-205-502 was used to localize dopamine D2 receptors by autoradiography after in vitro labeling of brain sections. The characteristics of the binding of this ligand to tissue sections were those expected from the labeling of dopamine D2 receptors. The binding of 3H-205-502 was inhibited selectively and stereospecifically by dopamine D2 a...

The goal of these studies was to define the rate-limiting steps in the inactivation of type 2 iodothyronine deiodinase (D2). We examined the effects of ATP depletion, a lysosomal protease inhibitor, and an inhibitor of actin polymerization on D2 activity in the presence or absence of cycloheximide or 3,3', 5'-triiodothyronine (reverse T3, rT3) in rat pituitary tumor cells (GH4C1). We also analy...

Prognostic factors for breast cancer include axillary lymph node status, tumor size, histology, nuclear grade, presence of estrogen and progesterone receptors, HER2/neu status, and mean microvessel density (MVD). In this study, we evaluated the usefulness of a new marker, D2-40, by investigating lymph vascular invasion of the tumor immunohistochemically in 132 patients with breast cancer and co...

The existence of two molecular forms of D2 dopamine receptors suggests that differences in the distribution or regulation of the two forms could be exploited for the pharmacological treatment of disease. Using probes selective for each alternatively spliced variant of D2 receptor mRNA, we determined that both variants were widely distributed in rat brain and pituitary but that the ratio of the ...