In Neisseria meningitidis, uridine, deoxyuridine, cytosine, cytidine, or deoxycytidine could not be used by uracil-requiring mutants as pyrimidine sources. Consistent with these findings, only 5-fluorouracil of the different fluoropyrimidine bases and nucleosides showed any inhibitory effect on the growth of four prototrophic strains of N. meningitidis. Likewise, only radioactive uracil was rea...

Degradation of uracil-containing DNA by Bacillus subtilis extracts and its inhibition after infection by the uracil-containing DNA phage PBS2 have been investigated to resolve differences between the published reports of Tomita and Takahashi (1975) and Friedberg et al. (1975, 1976). The product of hydrolysis of PBS2 DNA, tritium labeled in its uracil and cytosine residues, is solely uracil and ...

Deamination of cytosine to uracil is the most common promutagenic change in DNA, and it is greatly increased at the elevated growth temperatures of hyperthermophilic archaea. If not repaired to cytosine prior to replication, uracil in a template strand directs incorporation of adenine, generating a G.C --> A.U transition mutation in half the progeny. Surprisingly, genomic analysis of archaea ha...

aminofluorene for 90 days have a 10-fold increased capac ity to incorporate uracil into the ribonucleic acids in vivo (2, 16). According to current views (1, 6), the incorpora tion is determined by the activity of the anabolic as well as the catabolic enzyme systems involved in uradil metabo lism. The increased incorporation of uracil might thus be due, at least in part, to a decreased capacity...

aminofluorene for 90 days have a 10-fold increased capac ity to incorporate uracil into the ribonucleic acids in vivo (2, 16). According to current views (1, 6), the incorpora tion is determined by the activity of the anabolic as well as the catabolic enzyme systems involved in uradil metabo lism. The increased incorporation of uracil might thus be due, at least in part, to a decreased capacity...

Uracil, the pyrimidine nucleobase, which combined with adenine forms one of the major motifs present in the biopolymer RNA, is also involved in the self-assembly of RNA. In the title compound, C(9)H(14)N(4)O(2)·2H(2)O, the asymmetric unit contains one dimethyl-amino-uracil group and two water mol-ecules. The plane of the N=C-NMe(2) side chain is inclined at 27.6 (5)° to the plane of the uracil ...

The processing of a unique uracil in DNA has been studied in mammalian cells. A synthetic oligodeoxyribonucleotide carrying a potential Bgl II restriction site, where one base has been substituted with a uracil, was inserted in the early intron of SV40 genome. Various heteroduplexes were constructed in such a manner that the restitution of an active Bgl II restriction site corresponds in each c...

Ben-Ishai, Ruth (Israel Institute of Technology, Haifa), Michal Lahav, and Ada Zamir. Control of uracil synthesis by arginine in Escherichia coli. J. Bacteriol. 87:1436-1442. 1964.-It is shown that arginine affects uracil biosynthesis in Escherichia coli. The effect of arginine on uracil synthesis was analyzed by following the changes in the level of aspartate transcarbamylase, the first specif...

Facile and specific methods to quantify a nucleobase in biological samples are of great importance for diagnosing disorders in nucleic acid metabolism. In the present study, a novel fluorogenic reaction specific for uracil has been developed. The reaction was carried out in an alkaline medium containing benzamidoxime and K(3)[Fe(CN)(6)] which were heated for 2.0 min. Under the optimum reaction ...

Pritikin, William B. (University of California, Los Angeles), and W. R. Romig. Death of Bacillus subtilis auxotrophs due to deprivation of thymine, tryptophan, or uracil, J. Bacteriol. 92:291-296. 1966.-Auxotrophic mutants of Bacillus subtilis 168 that require either tryptophan, uracil, or thymine died rapidly when deprived of any of these compounds. Phage PBS1 was produced by infected B. subti...