Precise cellular localization of the GUS stain is notoriously difficult in Arabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol–acetic acid (EtAc) and Hoyer’s medium allows reliable cellular localization of the GUS, even in seeds from late developmental stages. This method also leads to the staining of nucleoli in the endospe...

The expression of the tobacco root-specific gene TobRB7 was characterized. Gel blot hybridizations to RNA isolated from various tobacco tissues demonstrated that steady-state TobRB7 mRNA is not detected in expanded leaf, stem, or shoot apex tissue. To determine the spatial pattern of expression, in situ hybridization to root sections revealed that TobRB7 expression is localized to root meristem...

0020-0255/$ see front matter 2010 Published b doi:10.1016/j.ins.2010.03.015 * Corresponding author. Tel.: +90 312 2901625; f E-mail addresses: [email protected] (E. D Get-Successors (GS) which retrieves all successors of a junction is a kernel operation used to facilitate aggregate computations in road network queries. Efficient implementation of the GS operation is crucial since the dis...

A helper-dependent expression system based on transmissible gastroenteritis coronavirus (TGEV) has been developed using a minigenome of 3.9 kb (M39). Expression of the reporter gene beta-glucuronidase (GUS) (2-8 microg per 10(6) cells) and the porcine respiratory and reproductive syndrome virus (PRRSV) ORF5 (1-2 microg per 10(6) cells) has been shown using a TGEV-derived minigenome. GUS express...

To learn more about the function and regulation of small heat shock proteins (sHSPs) during seed development, we studied sHSP expression in wild-type and seed maturation mutants of Arabidopsis by western analysis and using an HSP17.4 promoter-driven beta-glucuronidase (GUS) reporter gene in transgenic plants. In the absence of stress, GUS activity increases during development until the entire e...

Variation of transgene expression caused by either position effect at the insertion site or the promoter/enhancer elements employed for the expression of selectable marker genes has complicated phenotype characterization and caused misinterpretation. We have developed a reporter system in rice to analyze the influence of vector configuration, spacer and selectable marker gene promoter on the ex...

A polyubiquitin clone (ubi7) was isolated from a potato (Solanum tuberosum) genomic library using a copy-specific probe from a stress-induced ubiquitin cDNA. The genomic clone contained a 569-bp intron immediately 5' to the initiation codon for the first ubiquitin-coding unit. Two chimeric beta-glucuronidase (GUS) fusion transgenes were introduced into potato. The first contained GUS fused to a...

Guselkumab (GUS) studies in plaque psoriasis (PsO) have established a favorable safety profile for the drug. To evaluate cumulative experience PsO, we pooled placebo-controlled (wk 0-16 CNTO1959PSO2001, CNTO1959PSO3001/3002, and CNTO1959PSO3006) end-of-reporting 0-40 CNTO1959PSO3006; wk16-44 CNTO1959PSO3003; wk0-52 CNTO1959PSO2001 CNTO1959PSO3004; wk0-56 CNTO1959PSO3009; through wk264 CNTO1959P...