نتایج جستجو برای: روش mcr
تعداد نتایج: 371504 فیلتر نتایج به سال:
Since the initial discovery of mcr-1 in an Escherichia coli isolate from China, the gene has also been detected in Klebsiella pneumoniae and Salmonella enterica but is rarely reported in other Enterobacteriaceae Here, we report the isolation and identification of a Shigella flexneri strain harboring mcr-1 from stool samples in a pig farm in China from 2009. The MIC of colistin for the isolate i...
Methyl-coenzyme M reductase (MCR) is a nickel tetrahydrocorphinoid (coenzyme F430) containing enzyme involved in the biological synthesis and anaerobic oxidation of methane. MCR catalyzes the conversion of methyl-2-mercaptoethanesulfonate (methyl-SCoM) and N-7-mercaptoheptanoylthreonine phosphate (CoB7SH) to CH4 and the mixed disulfide CoBS-SCoM. In this study, the reaction of MCR from Methanot...
The origin and mobilization of the ~2,609-bp DNA segment containing the mobile colistin resistance gene mcr-1 continue to be sources of uncertainty, but recent evidence suggests that the gene originated in Moraxella species. Moreover mcr-1 can be mobilized as an ISApl1-flanked composite transposon (Tn6330), but many sequences have been identified without ISApl1 or with just a single copy (singl...
Mucormycosis (MCR) involving paranasal sinuses is a rare life-threatening opportunistic infection in immunocompromised individuals. In humans, MCR considered as one of the most rapidly progressive lethal forms fungal with high mortality rate 70–100%. During second wave COVID-19 India, cases have increased rapidly. We herein report case 70-year-old Indian male an uncontrolled diabetes diagnosed ...
Plasmid-mediated colistin-resistance genes have been reported worldwide in recent years. A multiplex polymerase chain reaction (Multi-PCR) protocol was developed to detect transferable colistinresistance ( mcr-1 mcr-6 ) Enterobacteria for clinical laboratory purposes.The authors first designed six new primer pairs amplify gene products achieve stepwise separation of amplicons between 87 216 bp,...
A temperature insensitive three-turn microfiber coil resonator (MCR) is demonstrated by embedding it in Teflon with opposite thermo-optic coefficient. The temperature dependence of a MCR strongly depends on the microfiber size which controls the ratio of thermal effect contributions from silica and polymer. Fabricated from a ~3μm-diameter microfiber, the temperature dependence of our MCR is com...
Methyl-coenzyme M reductase (MCR) from methanogenic archaea catalyzes the rate-limiting and final step in methane biosynthesis. Using coenzyme B as the two-electron donor, MCR reduces methyl-coenzyme M (CH3-SCoM) to methane and the mixed disulfide, CoBS-SCoM. MCR contains an essential redox-active nickel tetrahydrocorphinoid cofactor, Coenzyme F430, at its active site. The active form of the en...
We read with great interest the article in mBio regarding the discovery of a third colistin resistance determinant, mcr-3, in plasmid pWJ1 isolated from porcine Escherichia coli in China (1). This discovery and the subsequently reported discovery of a fourth colistin resistance determinant, mcr-4 (2), provide further support for our hypothesis that the mcr determinants (phosphoethanolamine tran...
The plasmid-mediated colistin resistance gene, mcr-1, was detected in an Escherichia coli isolate from a Danish patient with bloodstream infection and in five E. coli isolates from imported chicken meat. One isolate from chicken meat belonged to the epidemic spreading sequence type ST131. In addition to IncI2, an incX4 replicon was found to be linked to mcr-1. This report follows a recent detec...
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