In FRTL-5 cells, we and others have shown that TSH and insulin-like growth factor-I (IGF-I) stimulate DNA synthesis synergistically. The present study was undertaken to determine whether interaction between TSH and IGF-I also affects protein synthesis in this cell line, and if so by what mechanism. Quiescent cells were treated with TSH and/or IGF-I and [3H]valine incorporation into the acid-ins...

CONTEXT The regulation of TSH bioactivity in humans is not completely understood. OBJECTIVE The aim of the study was to investigate the role of serum thyroid hormones in regulating the bioactivity of TSH. DESIGN We determined in vitro TSH bioactivity and glycosylation in nine patients (six females and three males, age 41.3 yr) with primary hypothyroidism before and after L-T(4) replacement,...

PURPOSE This study was to evaluate the effect of neonatal, maternal, and delivery factors on neonatal thyroid-stimulating hormone (TSH) of healthy newborns. METHODS Medical records of 705 healthy infants born through normal vaginal delivery were reviewed. Neonatal TSH levels obtained by neonatal screening tests were analyzed in relation to perinatal factors and any associations with free thyr...

Thyrotropin (TSH) is an anterior pituitary glycoprotein hormone which modulates thyroid hormone production by the thyroid gland. TSH and its two subunits, TSH beta and alpha-subunit, are in turn regulated by the thyroid hormones and several stimulatory and inhibitory factors produced by the hypothalamus. Hypothalamic hormones such as thyrotropin-releasing hormone (TRH) and dopamine have profoun...

Bovine thyroid-stimulating hormone (thyrotropin, TSH) has been separated into two different subunits by gel filtration in ammonium bicarbonate solution after prior treatment with 1 M propionic acid. The two subunits, which have been designated TSH-a! and TSH-0, resemble each other more closely in amino acid composition than do the two subunits of luteinizing hormone (LH, interstitial cell-stimu...

Thymic hyperplasia is frequently observed in Graves' disease. However, detectable massive enlargement of the thymus is rare, and the mechanism of its formation has remained elusive. This case showed dynamic changes in thymic hyperplasia on serial computed tomography images consistent with changes in serum thyrotropin receptor (TSH-R) antibodies and thyroid hormone levels. Furthermore, the patie...

There are two main animal sources; rabbit antihuman TSH available from the National Pituitary Agency (NPA) or the Medical Research Council (MRC) and guinea-pig antihuman TSH prepared locally. Franchimont (1972) has also reported the use of antibodies to porcine TSH which crossreact with human TSH but this reagent is not generally available. Locally produced guinea-pig anti-TSH is used at a fina...

BACKGROUND Differences between the glycosylation patterns of a pituitary thyroid-stimulating hormone calibrator (pitTSH) and serum samples have been shown to be responsible for nonidentical epitope expression and for introducing discrepancies in TSH measurements. We studied the feasibility of developing new candidate reference materials by remodeling recombinant TSH (recTSH) to generate potenti...

This study investigated whether high-normal thyrotropin (TSH) levels are associated with metabolic syndrome in euthyroid Chinese people≥40 years old. Clinical and metabolic factors were assessed in 2,356 subjects (40-77 years old) with TSH levels in the normal range (0.35-5.00 mU/L). Using 2.50 mU/L as the cut-off point of TSH level within the normal range, we divided subjects into the high-TSH...