The aim of this study was to clarify whether myocardial cells with DNA fragmentation induced by ischemia show increased plasma-membrane permeability (PMP), focusing on the early lesions of acute myocardial ischemia (AMI). Rat AMI was induced by a permanent occlusion of the left coronary artery. The TUNEL method was used for the demonstration of DNA fragmentation, and lanthanum ions (La) were em...

The extent to which programmed cell death is the fate of proliferative, rather than post-mitotic, cells remains controversial, but a preponderance of evidence suggests that at least some cells within the brain's proliferative zone die during mammalian brain development. One major unresolved question is the extent to which cell death in the proliferative zone is spatiotemporally patterned. In or...

We examined the temporospatial pattern of naturally occurring apoptosis in chick embryos to five days of incubation (H.H. stages 1-25; Hamburger and Hamilton, 1951) using TUNEL labeling. The initial TUNEL-positive structure was the embryonic shield at stage 1. Apoptotic cells became ubiquitously present within embryos by stage 3, which is early in gastrulation. Until stage 6, TUNEL-positive cel...

BACKGROUND In this study we present the physiological changes observed in ejaculated spermatozoa of normospermic men after exposure to hydrogen peroxide (H(2)O(2)) or gamma irradiation. METHODS Motility changes as well as membrane and DNA-damage were determined in spermatozoa after incubation with 25 micromol/l of H(2)O(2) during increasing intervals of time (0--60 min and after 24 h) or afte...

به علت تغییرات حجم ناشی از خطای ساخت، فرکانس کاواک‌های شتاب‌دهی در شتاب‌دهنده‌های خطی الکترون تغییر می‌کند. نتیجه تنظیم فرکانسی کاواک‌ها یکی مراحل مهم تست سرد است که باید الزامات آن طراحی لحاظ شود. پروژه ساخت ساختار موج‌ایستا برای شتاب­دهنده با دو انرژی، پس طراحی، یک آلومینیمی (شامل نیم کاواک و تزویج) ساخته شد. این جبران پیچ‌های استفاده پیچ‌ها جزیی کاواک‌ها، آن‌ها را می‌دهند. مقاله محاسبات شبیه...

PURPOSE TUNEL assay is widely used to evaluate cell death. Quantification of TUNEL-positive (TUNEL+) cells in tissue sections is usually performed manually, ideally by two masked observers. This process is time consuming, prone to measurement errors, and not entirely reproducible. In this paper, we describe an automated quantification approach to address these difficulties. METHODS We develop...

BACKGROUND Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes have been reported to show apoptosis, as revealed by DNA fragmentation by DNA ladder and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted myocytes have apoptotic or o...