BACKGROUND Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within the...

The construction of a dense genetic map for Vitis vinifera and its anchoring to a BAC-based physical map is described: it includes 994 loci mapped onto 19 linkage groups, corresponding to the basic chromosome number of Vitis. Spanning 1,245 cM with an average distance of 1.3 cM between adjacent markers, the map was generated from the segregation of 483 SNP-based genetic markers, 132 SSRs, and 3...

Vitis vinifera vinifera is a hermaphrodite subspecies, while its ancestor, Vitis vinifera sylvestris, is dioecious. We have identified two genes that together allow the discrimination between male, female and hermaphrodite Vitis plants. The sex locus region on chromosome 2 was screened resulting in the discovery of a new gene, VviFSEX. The same screening revealed another gene, VviAPRT3, located...

Pollen germination of Vitis vinifera L., of the family Vitaceae was examined in fresh and stored pollen upto 48 weeks at different temperature i.e., refrigerator (+4C), freezer (-20C, -30C) and freeze drier (-60C). Pollen stored at low temperature showed better germination percentage as compared to pollen stored at +4C and fresh. Freeze dried pollen (-60C) showed the highest germination percent...

S. Afr. J. Enol. Vitic., Vol. 35, No. 2, 2014 *Corresponding author: [email protected] [Tel./fax: +86 10 62737553] Acknowledgements: This work was financially supported by the Natural Science Foundation of China (No. 31171786), the Special Fund for Agro-scientific Research in the Public Interest (No. 201303076-03) and the China Agriculture Research System (CARS-30) Separation and Purification of ...

Appearance of a lag period dependent on pH in the expression of the catecholase activity of a polyphenoloxidase extracted in a latent state from Airen grape (Vitis vinifera L.) berries, is revealed, suggesting the hysteretic nature of the enzyme. The lag time was independent of enzyme concentration, indicating that slow pH-induced conformational changes in the protein must occur during assay. R...

S u m m a r y : Isotope dilution assay was used for the qualitative and quantitative analysis of L-kynurenine and indole-3-acetic acid in grape must . After solid phase extraction, highest selectivity and sensitivity was achieved by high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) using selected reaction monitoring (SRM) . In the 24 sample...

Grapevine phylloxera, Daktulosphaira vitifoliae (Fitch) (Hemiptera: Phylloxeridae) is a worldwide pest of Vitis species. It has forms that feed on leaves and roots. Root forms predominate on Vitis vinifera (L.) cultivars, while leaf forms predominate on Vitis species from its native American range. Recently, high densities of D. vitifoliae infestations in leaves of V. vinifera in Brazil, Peru, ...