Efforts to express lipase in the periplasmic space of Escherichia coli have so far been unsuccessful andmost of the expressed recombinant lipases accumulate in the insoluble cell fraction. To evaluate the role ofnative and heterologous signal peptides in translocation of the lipase across the inner membrane of E. coli,the lipase gene (btl2) was cloned downstream of the native ...

escherichia coli (e. coli) bacteria can damage dna of the gut lining cells and may encourage the development of colon cancer according to recent reports. genetic switches are specific sequence motifs and many of them are drug targets. it is interesting to know motifs and their location in sequences. at the present study, gibbs sampler algorithm was used in order to predict and find functional m...

pigeons can be carriers of some human pathogens and contribute to transmission and spread of drug resistant infectious agents to human. considering many people’s interests to keep pigeons, present study was conducted to pcr detection of escherichia coli o157:h7 that is responsible for outbreaks of human intestinal diseases and fatal haemolytic-uraemic syndrome worldwide and evaluation of resist...

the aim of the present study was to evaluate the presence anti- e. coli, -rotavirus and -coronavirus, in calves' sera. a total of 184 calves under 1 month of age (84 diarrheic & 100 normal) was studied. serological tests including: direct elisa for detection of anti-k99 e. coli, - rotavirus and - coronavirus and tube agglutination test for detection anti-o157 e. coli, antibodies were used....

background trachyspermum ammi or carum copticum, commonly known as ajowan or ajwain is a plant used as a spice. thymol, the major phenolic compound present in ajowan is known as a strong germicide and anti-spasmodic. it is also used for cleaning the wounds and treating skin infections. objectives here, we aimed to study the inhibitory effects of ajowan essential oil on microorganisms like esche...

background and objectives: diarrheagenic escherichia coli (dec) strains are a major cause of intestinal syndromes in the developing countries. the aim of this study was to determine the prevalence of enterotoxigenic e. coli (etec) and enteroinvasive e. coli (eiec) in relation to phylogenetic background from patients with diarrhea. materials and methods: a total of 110 e. coli isolates were obta...

enterohemorrhagic escherichia coli (ehec), such as e. coli o157:h7, are emerging food-borne pathogens worldwide. this micro-organism can damage the epithelial tissue of the large intestine. the cytotoxic effects can be neutralized by probiotics such as bifidobacterium bifidum. probiotics are viable cells that have beneficial effects on the health of the host. the preventing activity of b. bifid...

Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum β-lactamases (ESBLs). Clear identification of risk factors for ESBL-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs patter...

  Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...