Diets that are high in dietary fiber are reported to have substantial health benefits. We sought to compare the metabolic effects of 3 types of dietary fibers -- sugarcane fiber (SCF), psyllium (PSY), and cellulose (CEL) -- on body weight, carbohydrate metabolism, and stomach ghrelin gene expression in a high-fat diet-fed mouse model. Thirty-six male mice (C57BL/6) were randomly divided into 4 ...

We present deep optical spectra of the archetypal young planetary nebula (PN) NGC7027, covering a wavelength range from 3310 to 9160 Å. The observations were carried out by uniformly scanning a long slit across the entire nebular surface, thus yielding average optical spectra for the whole nebula. A total of 937 emission features are detected. The extensive line list presented here should prove...

A novel stability-indicating reverse phase high performance liquid chromatography method was developed and validated for the simultaneous determination of Celecoxib (CEL) and Diacerein (DIN) and its impurities in capsule dosage form. The method was developed using L1 column with gradient using the mobile phase consist of Solution A (pH = 2.3 buffer) and Solution B (methanol and acetonitrile; 50...

Downlo ivated oncogenes are the dominant drivers of malignant progression in human cancer, yet little is known how the transformation from proto-oncogene to activated oncogene drives the expression of transd phenotypes. An isogenic model of HER-2–mediated transformation of human mammary epithelial as used along with HER-2–amplified human breast cancers to investigate how HER-2 activation alters...

We view the Internet as a “virtual laboratory” and have developed a framework to support experiments in web-based community learning. Our system is called the Community of Evolving Learners, or CEL. The target user group is primary school children, therefore we pay particular attention to privacy issues and provide a safe environment in which learners can succeed and fail incognito. This articl...

MicroRNAs (miRNA) are single-stranded 17- to 27-nucleotide RNA molecules that regulate gene expression by posttranscriptional silencing of target mRNAs. Here, we transformed rat 9L gliosarcoma cells to express cel-miR-67, a miRNA that lacks homology in rat. Coculture of these cells with cells that expressed a luciferase reporter that contained a complementary sequence to cel-miR-67 resulted in ...

Carboxyl ester lipase (bile salt-stimulated lipase) is a pancreatic enzyme capable of hydrolyzing esters of cholesterol and fat-soluble vitamins. It also efficiently digests triglycerides (TG) into free fatty acids and glycerol and is abundant in the milk of humans and several other species. We used the mouse as a model to test the hypothesis that milk-derived carboxyl ester lipase (CEL) digest...

The first formal classification of chronic myeloid neoplasms is credited to William Dameshek, who in 1951 described the concept of "myeloproliferative disorders (MPD)" by grouping together chronic myelogenous leukemia, polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). The 2001 World Health Organization (WHO) classification of myeloid malignancies included ...