The dithiol reagents phenylarsine oxide (PAO) and dibromobimane (DBrB) have opposite effects on the F 1 O -ATPase activity. PAO 20% increases ATP hydrolysis at 50 μM when enzyme activity is activated by natural cofactor Mg 2+ 150 it Ca . PAO-driven activation reverted to basal dithiothreitol (DTE). Conversely, 300 DBrB decreases 25% 50% In both cases, inhibition insensitive DTE. mitochondrial p...

IBMPFD/ALS is a genetic disorder caused by single amino acid mutation on the p97 ATPase, promoting ATPase activity and cofactor dysregulation. The disease mechanism underlying malfunction remains unclear. To understand how alters regulation, we assembled full-length p97R155H with its p47 first visualized their structures using single-particle cryo-EM. More than one-third of population was dodec...

Despite their rigidity, microtubules in living cells bend significantly during polymerization resulting in greater curvature than can be explained by thermal forces alone. However, the source of the non-thermal forces that bend growing microtubules remains obscure. We analyzed the motion of microtubule tips in NIH-3T3 fibroblasts expressing EGFP-EB1, a fluorescent +TIP protein that specifically...

During periods of crisis, cells must compensate to survive. To this end, may need alter the subcellular localization crucial proteins. Here, we show that during starvation, VCP, most abundant soluble ATPase, relocalizes and forms aggregate-like structures at perinuclear regions in PC3 prostate cancer cells. This movement is associated with a lowered metabolic state, which mitochondrial activity...

all species in the genus lysiphlebus förster (hymenoptera: braconidae, aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. a pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, l. fabarum marshall, collected from guilan province (iran)....

background: antifungal drug resistance and few numbers of available drugs limit therapeutic options against fungal infections. the present study was designed to discover new antifungal drugs.   m aterials and methods: this study was carried out in two separate steps, that is, in silico lead identification and in vitro assaying of antifungal potential. a structural data file of a ternary complex...

Despite prophylaxis and attempts to select a therapy, the frequency of preeclampsia does not decrease it still takes leading position in structure maternal mortality morbidity worldwide. In this review, we present new theory etiology pathogenesis that is based on interaction Na/K-ATPase its endogenous ligands including marinobufagenin. The signaling pathway marinobufagenin involves an inhibitio...

We review here briefly the essentials of vertebrate cardiac pacemaking system consisting populations ion channels that drive heartbeat. In addition to sarcolemmal system, there is a second channel-based oscillator centrally reciprocating Ca2+ flow between sarcoplasmic reticulum and cytosol regulated by release through ryanodine receptors active return Sarcoplasmic/Endoplasmic Reticulum Calcium ...