We describe a novel assay for detection of point mutations. The method combines the specificity and sensitivity of the potymerase chain reaction (PCR) and allele-specific oligonucleotides (ASO) with highly sensitive timeresolved fluorometry. ASO probes differing by a single base substitution and labeled with europium (Eu) chelates were hybridized in solution simultaneously with a biotinylated o...

Atrial septal defect (ASD) is one of the most common types congenital heart defects. Percutaneous closure ASD relatively safe and accepted as an alternative to surgery. However, device-related complications can occur rarely. Embolization Amplatzer atrial occluder (ASO) major complication percutaneous device closure. The author presents a case early embolization ASO into left atrium, which remov...

BACKGROUND AND OBJECTIVES Although coronary artery obstruction, aortic insufficiency (AI), and pulmonary stenosis (PS) have been reported after arterial switch operation (ASO), limited long-term studies on ASO exist. Our study aimed to examine long-term outcomes after ASO for simple complete transposition of the great arteries (TGA). SUBJECTS AND METHODS All 108 patients with simple complete ...

OBJECTIVES Myocardial blood flow (MBF) in children late after arterial switch operation (ASO) was investigated quantitatively by positron emission tomography (PET). BACKGROUND In children with transposition of the great arteries (TGA), ASO is widely accepted as the management of choice. The long-term patency of coronary arteries after surgical transfer to the neo-aorta, however, remains a con...

Differences in sensitivity of monkeys and humans to antisense oligonucleotide (ASO)-induced complement alternative pathway (AP) activation were evaluated in monkeys, humans, and in serum using biochemical assays. Transient AP activation was evident in monkeys at higher doses of two 2'-O-methoxyethyl (2'-MOE) ASOs (ISIS 426115 and ISIS 183750). No evidence of AP activation was observed in humans...

A 3.5-year-old girl (weight, 19 kg; height, 103 cm) was hospitalized for interventional closure of secundum atrial septal defect (ASD II). In transthoracic and transesophageal echocardiography (TEE), we found a hemodynamically relevant left-to-right shunt caused by isolated ASD II without any systemic or pulmonary venous anomaly. All heart valves were patent and showed no regurgitation. The siz...

Background Patients who received orthodontic treatment are likely to have apical root shortening. It appears that external apical root resorption results from a combination of patient-related risk factors such as genetic influences, systemic factors, and orthodontic treatment-related factors. Regarding the fact that the anterior segmental osteotomy (ASO) has been known for its possibility of co...

OBJECTIVE Long-term angiographic evaluation of left ventricular performance and size of the great arteries after one-stage neonatal versus two-stage arterial switch operation (ASO) of simple transposition. METHODS Analysis of cineangiographic studies obtained during the process of two-stage ASO for 34 patients and after neonatal repair for 52 patients. RESULTS At early follow-up after two-s...

Rapid two-stage arterial switch operation (ASO) is very relevant as many patients of transposition of great arteries (TGA) present late to the hospital when primary switch either is not possible or carries a high risk of morbidity and mortality. Hence, other means apart from the traditional methods of left ventricle preparedness should be tried to help this category of patients, who are to unde...

Since 1935 several reports have dealt with the antistreptolysin-O (ASO) titre in rheumatoid arthritis (RA). The mean and the upper ASO values were first established in a group of normal subjects and in patients with no history of recent streptococcal illness, and subsequently the distribution of the ASO titres was recorded in patients suffering from RA. Table I summarizes the results of Blair a...