In the recent years, laser ablation in liquid has become an increasingly important technique for the fabrication of NPs. this paper reports our recent studies on the generation of ZnO NPs by ablation of metal targets in aqueous environments using Q-switch Nd-YAG laser (λ=532nm) immersed in NaOH (0.1M). The Surface topography studied by atomic force microscopy revealed wider size distributio...

An autonomous surface vehicle instrumented with optical and acoustical sensors was deployed in Kane’ohe Bay, HI, U.S.A., to provide high-resolution, in situ observations of coral reef reflectancewithminimal human presence. The data represented awide range in bottom type, water depth, and illumination and supported more thorough investigations of remote sensing methods for identifying and mappin...

The replication of herpes simplex virus type 1 (HSV-1) DNA is associated with a high degree of homologous recombination. While cellular enzymes may take part in mediating this recombination, we present evidence for an HSV-1-encoded recombinase activity. HSV-1 alkaline nuclease, encoded by the UL12 gene, is a 5'-->3' exonuclease that shares homology with Redalpha, commonly known as lambda exonuc...

Cell reprogramming requires efficient delivery of reprogramming transcription factors into the cell nucleus. Here, we compared the robustness and workload of two protein delivery methods that avoid the risk of genomic integration. The first method is based on fusion of the protein of interest to a protein transduction domain (PTD) for delivery across the membranes of target cells. The second me...

We have developed a selective upconversion switching method for the ratiometric fluorescence detection of nitrite using upconversion nanoparticles (UCNPs) and an efficient nitrite reaction. The green emission (λ(em) = 539 nm) of NaYF4:Yb(3+),Er(3+) nanoparticles can be selectively quenched by the neutral red (NR) dye due to the spectral overlap between the emission at 539 nm and the absorption ...

An attempt was made to knockout the Escherichia coli C29 lacI gene with a kanamycin resistance cassette isolated from the plasmid pACYC177. This process used the lambdared recombinase system to allow for homologous recombination, and subsequent insertion of the kanamycin cassette into the lacI gene. The E. coli C29 cells were initially transformed with pKD46, which contains the lambda-red recom...

We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda (lambda) bet or Escherichia coli recT. Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the nine other genes when expressed in E. coli carries out oligonucleotide-mediated recombination. To o...