We investigated the combined effects of microbial transglutaminase (MTGase, 7.0 units/mL) and microwave irradiation (MI) on the polymerization of milk proteins at 30 °C for 3 h. The addition of MTGase caused the milk proteins to become polymerized, which resulted in the formation of components with a higher molecular-weight (>130 kDa). SDS-PAGE analysis revealed reductions in the protein conten...

Numerous statistical machine learning methods suitable for application to highly correlated features, as those that exist spectral data, could potentially improve prediction performance over the commonly used partial least squares approach. Milk samples from 622 individual cows with known detailed protein composition and technological trait data accompanied by mid-infrared spectra were availabl...

The nucleotide sequences corresponding to bovine crs2and p-casein mRNAs have been determined by cDNA analysis. Both sequences appear to be complete at their 5’ ends. The nucleotide sequence of us2-casein, when compared with the corresponding cavine A sequence, helps to define the boundaries of a large amino acid repeat (-80 residues) whereas comparisons with the nucleotide sequences of rat yand...

At the heart of the milk system are the colloidal casein-calcium-transport complexes termed the casein micelles. The application of physical chemical techniques such as light, neutron, and X-ray scattering, and Electron Microscopy (EM) has yielded a wealth of experimental detail concerning the structure of the casein micelle. From these experimental data bases have arisen two conflicting models...

The aim of this study is to reveal the effect Lepidium sativum L. on ripening characteristics White cheese. A control cheese without garden cress and cheeses with four different concentrations (0.6-1.2-1.8-2.4%) were produced their dry matter, fat, protein contents; pH titratable acidity levels; water-soluble nitrogen (WSN) trichloroacetic acid-soluble (TCA-SN) determined electrophoretograms sh...

β-Lap prodrug micelle strategy improves the formulation properties of β-lap therapeutics. The resulting micelles yield apparent high β-lap solubility (>7 mg mL(-1) ), physical stability, and ability to reconstitute after lyophilization. In the presence of esterase, β-lap prodrugs are efficiently converted into parent drug (i.e., β-lap), resulting in NQO1-dependent lethality of NSCLC cells.

The quantification of allergens in food including baked food matrices is of great interest. The aim of the present study was to describe a non-immunologic method to quantify bovine β-casein using ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-TQ-MS/MS) in multiple reaction monitoring (MRM) mode. Eight of 10 theoretical peptides from β-casein after trypt...