SUMMARY The drugs [1-ß-D-arabinofuranosylcytosine (ara-C), hy-droxyurea (HU), 5-hydroxy-2-formylpyridine thiosemicar-bazone (5-HP), and camptothecin sodium salt (camptothecin)] considered in this paper markedly inhibit DNA synthesis and are maximally cytotoxic to cells in S phase. In these studies, high-specific-activity thymidine-3H (HSA-TdR-3H) was used as a control compound which killed cel...

Mitochondria from regenerating liver of normal and alloxan-diabetic rats were assayed polarographically for respi ratory rate and oxidative phosphorylation. Approximately 65 to 70% of the liver was temoved by a simple surgical procedure, and the animals were sacrificed at different intervals from 16 hr to 4 weeks after partial hepatectomy. Mitochondria were isolated in a sucrose/tris(hydroxymet...

Seven isozymes of cytochrome P-450 were tested to establish whether they could W-oxidize azoprocarbazine to form the two isomerie azoxy metabolites after optimizing the reconstitution of various purified isozymes with regard to substrate concentration, exogenous lipid, and reduced nicotinamide adenine dinucleotide phosphate-cytochrome c (P-450) reducÃ-aseconcentration. Two isozymes, cytochromes...

The recently discovered tumor suppressor gene maspin has been shown to inhibit tumor cell motility, invasion, and metastasis in breast cancer by our laboratories. Nonetheless, the exploitation of maspin as a potential diagnostic and/or therapeutic tool has remained limited due to the lack of knowledge concerning its molecular and biological mechanism(s) of ac tion. The work reported here demons...

Shionogi carcinoma 115 (SCI 15) has been accepted for 20 years as an androgen-responsive mouse mammary tumor. In a serum-free culture system we have established (Ham's F-l2:Eagle's minimum essential medium (1:1, v/v) containing 0.1% bovine serum albumin), 10 *M testos terone markedly stimulates the growth of SC-3 cells (a cloned cell line from a SCI 15 tumor) via androgen receptor. The testoste...

The effect of fatty acid structure on utilization by Ehrlich ascites tumor cells was studied in vitro. Unesterified palmitate, stéarate, oleate, and linoleate were metabolized in a qualitatively similar fashion and at roughly comparable rates. Each of these fatty acids was rapidly taken up by the cells in unesterified form. The amount of free fatty acid taken up during the first few minutes wa...

Peripheral blood lymphocytes cultured in recombinant interleukin 2 during 3 to 5 days (short-term cultures) develop the ability to lyse natural killer-resistant tumor lines and fresh tumor cells, i.e., express lymphokine-activated killer (LAR) function. Phenotypic analysis has shown these cells to be natural killer cells, i.e., CD16+ and/or Leu 19+ cells. CD3*,CD16~ T-cells, instead, develop ve...

The acute effect of RNA and DNA synthesis inhibitors on DNA topoisomerase (topo) I localization within cells was examined. Indirect imiminofluorescence revealed that topo I was distributed throughout the nuclei hut was concentrated in nucleoli of untreated K562 leukemia cells and A549 non-small cell lung cancer cells. Treatment with the DNA polymerase inhibitor aphidicolin did not alter this di...

Exposure of newborn and infant mice to urethan results in a significantly higher incidence of hepatomas in male mice than in females. Sex of the animals, however, does not influence the development of malignant lymphomas, Harderian gland tumors, and lung adenomas. In the present study, the role of gonads in the development of urethan-induced hepatomas and other tumors has been investigated. Sev...

Proper implementation of direct injection clearance techniques to measure tumor blood flow (TBF) requires knowledge of the tracer distri bution because TBF distribution is often inhomogeneous. Therefore, deuterium nuclear magnetic resonance imaging was used to follow tracer (HOD) distribution after direct injection of 10-40 ß\ isotonic saline/D2O into RII -I tumors. Within 2 to 4 min after int...