سید عباس شجاع الساداتی
استاد، دانشگاه تربیت مدرس، دانشکدۀ مهندسی شیمی، گروه بیوتکنولوژی
[ 1 ] - بررسی مخاطرات نانوذرات مهندسی‌شده (ENPs)
با وجود گسترش و پیشرفت سریع کاربردهای نانوفناوری، تحقیقات بسیار اندکی در زمینۀ ارزیابی و اندازه‌گیری خطرهای نانوذرات در سامانه‌های زیستی و بوم‌سازگان اجرا شده است. اندازۀ کوچک نانوذرات و خواص ویژۀ آنها سبب شده است که آنها یک حامل برای اتصال و انتقال آلوده‌کننده‌های شیمیایی سمی باشند. مطالعات زیادی نشان می‌دهد که نانوذرات و نانولوله‌ها قابل رهایش در محیط و مسبب آثار مضر برای انسان یا موجودات زند...
[ 2 ] - Controlled Green Synthesis of Silver Nanoparticles Using Culture Supernatant of Filamentous Fungus
The focus of this study was to evaluate the effects of some parameters influencing the size and size distribution of the silver nanoparticles (AgNPs) produced by culture supernatant of Fusarium oxysporum. Results revealed that in the reaction solution containing equal volume of silver nitrate and culture supernatant; pH, temperature, and light source can control the AgNP’s characteristics. ...
[ 3 ] - Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized
This study involves the isolation and characterization of a bacterial strain capable of bioleaching molybdenum ore. Bacterial growth was observed when rock sample was incubated in 9K at 70 ºC. The isolates were identified as extremely acidophilic, thermophilic and chemolithotroph archaebacteria. Following PCR amplification of the 16S rDNA of the isolated strain, the sequencing of this region an...
[ 4 ] - Optimization and characterization of Aspirin Encapsulated nano-Liposomes
Resistance to aspirin and its cytotoxicity significantly limits its therapeutic applications. Nano-liposomal encapsulation of aspirin can reduce its cytotoxicity. In this study, aspirin encapsulated nano-liposomes (AS-NL) was prepared and its performance in drug delivery and cytotoxicity was evaluated. The effects of two independent variables including: number of freeze/thawing cycles and conce...
[ 5 ] - Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles
Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...
[ 6 ] - Maximizing Production of Human Interferon-γ in HCDC of Recombinant E. coli
Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....
[ 7 ] - Simultaneous loading of 5-florouracil and SPIONs in HSA nanoparticles: Optimization of preparation, characterization and in vitro drug release study
Objective(s): Over the past two decades, considerable interest has been focused on utilizing biocompatible magnetic nanoparticles (MNPs) for biomedical applications. In this study, production of human serum albumin (HSA) nanoparticles using desolvation technique that were simultaneous loaded with high amounts of superparamagnetic iron oxide nanoparticles (SPIONs) and 5-flourouracil (5-FU) was i...
[ 8 ] - Optimization and characterization of Aspirin Encapsulated nano-Liposomes
Resistance to aspirin and its cytotoxicity significantly limits its therapeutic applications. Nano-liposomal encapsulation of aspirin can reduce its cytotoxicity. In this study, aspirin encapsulated nano-liposomes (AS-NL) was prepared and its performance in drug delivery and cytotoxicity was evaluated. The effects of two independent variables including: number of freeze/thawing cycles and conce...
[ 9 ] - Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles
Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...
[ 10 ] - Maximizing Production of Human Interferon-γ in HCDC of Recombinant E. coli
Tuning recombinant protein expression is an approach which can be successfully employed for increasing the yield of recombinant protein production in high cell density cultures. On the other hand, most of the previous results reported the optimization induction conditions during batch and continuous culture of recombinant E. coli, and consequently fed-batch culture have received less attention....
[ 11 ] - بهینه‌سازی تولید بیواتانول از باگاس نیشکر پیش تیمار اسیدی شده با به کارگیری پیکیا استیپیتیس
مقدمه: به علت محدود بودن سوخت‌های فسیلی و مصرف روز افزون آنها، تولید سوخت‌های زیستی مایع از اهمیت بالایی برخوردار است. استفاده از مواد اولیهای که مصرف غذایی نداشته باشند، مانند مواد لیگنوسلولزی برای تولید سوخت‌های زیستی نسل دوم مطرح‌اند. با توجه به اینکه مقدار در خور توجهی از مواد لیگنوسلولزی را قندهای پنتوز تشکیل می‌دهد، استفاده از این قندها برای تولید سوخت‌های زیستی اهمیت ویژهای...
[ 12 ] - بررسی عملکرد راکتور زیستی چکنده تلقیح‌شده با باکتری برای حذف بخار تولوئن
ز بین روش‌های گوناگونی که برای حذف مواد آلی فرار از هوای صنایع آلاینده محیط‌زیست وجود دارد، روش‌های زیستی به دلیل صرفه اقتصادی، کارایی بالا برای غلظت‌های کم و بدون تولید پسماند ثانویه موردتوجه هستند. استفاده از راکتورهای زیستی چکنده یکی از شناخته‌شده‌ترین روش‌های زیستی برای حذف ترکیب‌های آلی فرار از هوا به شمار می‌رود. بررسی پایداری عملکرد راکتورهای زیستی چکنده در طول زمان از اهمیت ویژهای ...
[ 13 ] - ابداع روشی کم‌هزینه و دقیق برای خالص‌سازی فاکتور رشد اپیدرمی انسانی نوترکیب (hEGF)
فاکتور رشد اپیدرمی(EGF) انسانی با 53 اسید آمینه و وزن مولکولی 2/6 کیلو دالتون، پروتئینی با قابلیت تحریک رشد چندین نوع سلول است و به دلیل همین ویژگی در موارد پزشکی و تهیه فراورده های آرایشی کاربردهای بسیاری دارد. در این پژوهش، ابتدا پلاسمید pET23a(+) حاوی ژن EGF به باکتری اشرشیاکلی سویه BL21(DE3) منتقل شد که سویه نوترکیب به دست آمده قادر به تولید فاکتور رشد اپیدرمی انسانی به صورت درون‌ریز ب...
[ 14 ] - ارزیابی باکتریهای باسیلوس سابتیلیس، اسینتوباکترکالکواسیتیکوس و سودوموناس در جداسازی نامیزه آب - نفت
تولید نامیزه آب ـ نفت یکی از مشکلات عمده در صنعت نفت محسوب میشود. در سالهای اخیر، روش‌های زیستی توجه زیادی را در جداسازی نامیزه آب و نفت به خود جلب کرده اند. در این پژوهش، سه سویه باکتریایی باسیلوس سابتیلیس، اسینتوباکتر کالکواسیتیکوس و سودوموناس برای جداسازی نامیزه به کار گرفته شدند.مایع های فوقانی به دست آم...
[ 15 ] - اصلاح و بهینه سازی فرایند استخراج کیتین از پوست میگو
در این پژوهش استخراج کیتین از پوسته میگو طی فرایندهای املاح زدایی و پروتئین زدایی از نظر زمان اقامت، غلظت مواد فراوری کننده یعنی هیدروکلریک اسید و سدیم هیدروکسید و دمای واکنش، بهینه سازی شد. در برخی از روش‌های موجود مرحله پروتئین زدایی مقدم بر املاح زدایی است، ولی آزمایش‌های انجام شده بیانگر این واقعیت است که فرایند املاح زدایی باید مقدم بر پروتئین زدایی باشد. مطابق آزمایش‌های انجام...
[ 16 ] - Enhancement of Alpha 1-antitrypsin Production in Pichia pastoris by Designing and Optimizing Medium Using Elemental Analysis
Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a ...
[ 17 ] - Optimization of RGD-modified Nano-liposomes Encapsulating Eptifibatide
Background: Eptifibatide (Integrilin) is an intravenous (IV) peptide drug that selectively inhibits ligand binding to the platelet GP IIb/IIIa receptor. It is an efficient peptide drug, however has a short half-life. Therefore, antithrombotic agents like eptifibatide are required to become improved with a protected and targeted delivery system such as using nano-liposomes to the site of thrombu...
[ 18 ] - The Effects of Organic Solvents on the Physicochemical Properties of Human Serum Albumin Nanoparticles
Background: Recently, applications of albumin nanoparticles as drug delivery carriers have increased. Most toxicology studies have shown that surface chemistry and size of nanoparticles play an important role in biocompatibility and toxicity. Objective: The effect of desolvating agents with different chemical properties on the size of synthesized HSA NPs was investigated. Materials and Method...
[ 19 ] - Evaluation of Cell Penetrating Peptide Delivery System on HPV16E7 Expression in Three Types of Cell Line
Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...
[ 20 ] - Recent Advances in High Cell Density Cultivation for Production of Recombinant Protein
This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...
[ 21 ] - Evaluation of Strategies for Temperature and Moisture Control in Solid State Packed Bed Bioreactors
Different control strategies of bed temperature and moisture were investigated using various inlet air temperatures and air fluxes in both the ordinary packed bed bioreactor (without cooling water in the jacket) and the bioreactor with cooling water in jacket. The experiments were carried out within a 1-L solid-state packed bed bioreactor in which Aspergillus niger was cultivated on wheat bran....
[ 22 ] - Optimization of factors affecting on sulfide oxidation from synthetic spent caustic by Haloalkaliphilic Thioalkalivibrio versutus by focus on sodium ion effect: Application of response surface methodology
In the present study, the effects of four factors including initial sulfide concentration (mg l-1 ), agitation speed (rpm), amount of inoculums (%) and sodium concentration (mg l-1) on removal efficiency (%R) and yield of sulfate production by Thioalkalivibrio versutus from synthetic spent caustic were investigated. For this purpose, experiments are designed by design of experiments (DOE) and R...
[ 23 ] - Keratin nanoparticles: synthesis and application for Cu(II) removal
A straightforward procedure to synthesize keratin nanoparticles (KNP) from chicken feathers was introduced. The characterization of the synthesized nanoparticles was done using Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD) patterns and transmission electron microscopy (TEM). The FTIR analysis revealed no significant chemical change after...
[ 24 ] - Application of a nanoporous metal organic framework based on iron carboxylate as drug delivery system
In the present study, a nanoporous metal organic framework (MOF) based on iron metal and amino terephthalate ligand MIL-101-NH2-Fe has been used as a carrier for loading and in vitro release of 5-flurouracil (5-FU) anticancer drug. The 5-FU drug loaded MOF was 13 wt % by using thermogravimetric analysis (TGA). The 5-FU release was monitored under physiological condition at 37°C, pH 7.4 in simul...
[ 25 ] - Application of a nanoporous metal organic framework based on iron carboxylate as drug delivery system
In the present study, a nanoporous metal organic framework (MOF) based on iron metal and amino terephthalate ligand MIL-101-NH2-Fe has been used as a carrier for loading and in vitro release of 5-flurouracil (5-FU) anticancer drug. The 5-FU drug loaded MOF was 13 wt % by using thermogravimetric analysis (TGA). The 5-FU release was monitored under physiological condition at 37°C, pH 7.4 in simul...
[ 26 ] - Bioconversion and Enzymatic Activities of Neurospora Sitophila Grown Under Solid State and Submerged Fermentation on Sago Hamps (RESEARCH NOTE)
N.sitophila was grown under controlled conditions of solid state and submerged fermentation on Sago hampas. The optimum conditions of protein enrichment previously established for sugar beet pulp was used for this study. Under this condition the protein content of Sago hampas under solid state increased from 1.4 to 14.45% (W/W) whereas for Sago hampas and Sago starch, the protein content under ...
[ 27 ] - بررسی و مقایسه برنامه درسی و شیوه آموزشِ رشته مهندسی شیمی در دانشگاه‌های آمریکا و ایران
فناوری امروزه بشر را می‌توان مدیون تلاش، نوآوری و خلاقیت مهندسان دانست. مهندسان برآنند تا هر روزه با کشف راه‌ها و ابزارهای جدید و پیشرفته، زندگی را بر انسان آسان‌تر کنند. تأثیر بسزای برنامه درسی دانشگاهها بر توانایی‌های دانشآموختگان مهندسی و سرعت فناوری عصر حاضر ایجاب می‌کند که برنامه درسی دانشگاهها بهصورت مداوم ارزیابی و در صورت لزوم، کمبودها و نقاط ضعف آن رفع و تصحیح شود. بدین منظور، می‌ت...
[ 28 ] - RGD-Modified Nano-Liposomes Encapsulated Eptifibatide with Proper Hemocompatibility and Cytotoxicity Effect
Background: Eptifibatide (Integrilin®) is a hepta-peptide drug which specifically prevents the aggregation of activated platelets. The peptide drugs are encapsulated into nanolipisomes in order to decreasing their side effects and improving their half-life and bioavailability. Objectives: In this study, the in vitro cytotoxicity and hemocompatibi...
[ 29 ] - Evaluation of Ethanol Production from Tannin-Reduced Carob Pod Extracts by Zymamonas Mobilis
Regarding some reported antimicrobial properties of tannins; Zymomonas mobilis was used to obtain ethanol from tannin-reduced carob pod extract (TR-CPE). Culture of 50 mL volume containing 7.5 g sugar at pH 5.5 and 0.03 g bacterial inoculums with shaking at 80 rpm was used. Using response surface methodology (RSM), the maximum ethanol concentration of 5.34 % w/v (higher than that reported earli...
[ 30 ] - FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration
Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...
[ 31 ] - FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration
Recent studies suggest that reducing the induction temperature would improve the quality of some recombinant inclusion bodies (IB) by providing a native-like secondary structure and leading to an improvement in protein recovery. This study focused on optimizing the solubilization condition of Reteplase, a recombinant protein with 9 disulfide bonds. The influence of lowering induction temperatur...
نویسندگان همکار
محسن آهی 1