زهرا مشکات
دانشیار ویروس‌شناسی، مرکز تحقیقات ویروس شناسی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.
[ 1 ] - بررسی ارتباط بین مورفه آ و سیتومگالوویروس در یک مطالعه مقطعی
مقدمه اسکلرودرمی موضعی، جزئی از طیف بیماریهای اسکلروتیک است که به طور اولیه پوست را تحت تاثیر قرار میدهد و شایعترین آنها مورفه آ میباشد. تا به حال علل و پاتوژنز مورفه آ به طور کامل شناخته نشده است اما فرض بر این است که یک فرایند چند عاملی باعث شروع آبشار التهابی و فیبروتیک میشود. عوامل محیطی احتمالی دخیل شامل بیماری لایم، تروما، پرتوتابی، داروها و عفونتهای ویروسی است که از میان عوامل ...
[ 2 ] - نانو ذرات در تشخیص و درمان عفونت هپاتیت C
عفونت هپاتیت C یکی از اصلی‌‌ترین مشکلات بهداشتی جهان است. دو سوم جمعیت جهان یعنی حدود ۱۷۰ میلیون نفر به عفونت مزمن هپاتیت C مبتلا هستند و هر ساله هزینه‌های فراوانی صرف تشخیص و درمان این بیماری می شود. ویروس هپاتیت C ویروسی با تمایل به ایجاد عفونتهای مزمن (۷۰ تا ۸۰ درصد موارد ) است که در فاز انتهایی به سیروز یا سرطان کبد منجر می گردد. به دلیل وجود جهش‌های فراوان در ژنوم این ویروس، امکان تول...
[ 3 ] - کاربرد نانوساختارهای تشخیصی و درمانی در سرطان گردن رحم: یک مقاله مروری
مقدمه: سرطان، یکی از اصلی‌‌ترین مشکلات سلامتی انسانها در سراسر جهان است. از این میان، سرطان گردن رحم دومین سرطان شایع در میان زنان در دنیاست. حدود ۸۰% از موارد جدید سرطان گردن رحم در کشورهای در حال توسعه اتفاق می‌افتد. با توجه به مشکلات موجود در امر تشخیص و درمان این سرطان طی دو دهه گذشته، دارو‌های مولکولی، کمک شایانی به تشخیص و درمان انواع سرطانها از جمله سرطان گردن رحم نموده‌اند که از ج...
[ 4 ] - کلونیزاسیون استرپتوکوکوس آگالاکتیه در زنان باردار ایران: مرور نظام‌مند
مقدمه: استرپتوکوکوس آگالاکتیه می‌تواند در رکتوواژینال زنان باردار کلونیزه شده و با انتقال به نوزاد، منجر به بیماریهای زودرس نوزادی خطرناک مانند سپسیس و پنومونی شود. برای پیشگیری از این عوارض باید پروفیلاکسی آنتی‌بیوتیکی در مادران باردار حامل باکتری انجام شود. آمارهای متفاوتی از میزان کلونیزاسیون این باکتری در ایران وجود دارد و میزان شیوع کلی آن مشخص نیست. مطالعه مروری حاضر با هدف بررسی میزان ک...
[ 5 ] - بررسی عفونت سل در زنان باردار و اثرات آن بر نوزادان
مقدمه: باکتری مایکوباکتریوم توبرکلوزیس، عامل بیماری سل، به عنوان یک تهدید فراگیر جوامع شناخته می شود. بر اساس گزارش سازمان جهانی بهداشت در سال 2014، حدود 3/3 میلیون زن باردار عفونت فعال سل داشته اند. این میزان بالای ابتلاء، چالش بزرگی برای بهداشت جوامع محسوب می شود. پیشگیری و کنترل سل در زنان باردار به دلیل وضعیت خاص سیستم ایمنی، نیازمند تدابیر خاص است. لذا مطالعه مروری حاضر با هدف بررسی اثرات ...
[ 6 ] - بررسی فراوانی عفونت کلامیدیا تراکوماتیس در نمونههای مجرای ادرار و دهانه گردن رحم ارسالی به بیمارستان قائم (عج) مشهد
مقدمه: کلامیدیا تراکوماتیس قادر است دستگاه تناسلی را آلوده کند و عامل ایجاد طیف وسیعی از تظاهرات بالینی در زنان شود که از عفونتهای بدون علامت تا عفونتهای پیش‌رونده پیچیده مانند بیماریهای التهابی لگن، نازایی و حاملگی نابه‌جا متغیراست. همچنین در مردان کلامیدیا شایعترین عامل ترشحات مجرا است. هدف از این مطالعه تعیین فراوانی عفونت کلامیدیا در نمونههای مجرا و گردن رحم بیماران مراجعه‌کننده به بیم...
[ 7 ] - A study on the immune response induced by a DNA vaccine encoding Mtb32C-HBHA antigen of Mycobacterium tuberculosis
Objective(s): Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses...
[ 8 ] - Current progress in the development of therapeutic vaccines for chronic hepatitis B virus infection
Chronic hepatitis B is still a major public health issue despite the successful prophylactic vaccination attempts. Chronicity of hepatitis B virus(HBV) is mainly due to its ability to debilitate host's immune system. Therefore, major measures have been taken to stop this process and help patients with chronic hepatitis B infection recover from their illness. While satisfactory results have been...
[ 9 ] - The Prevalence of TEM and SHV Genes among Extended-Spectrum Beta-Lactamases Producing Escherichia Coli and Klebsiella Pneumoniae
Objective(s) Production of extended-spectrum beta-lactamases (ESBLs) by enteric bacteria continues to be a major problem in hospitals and community. ESBLs producing bacteria cause many serious infections including urinary tract infections, peritonitis, cholangitis and intra-abdominal abscess. The aim of this study was to determine the prevalence of ESBLs producing Escherichia coli and Klebsiel...
[ 10 ] - Development of a Western Blot Assay for Detection of Antibodies against HSV Using Purified HSV Virions Prepared by Sucrose Density Gradient
Objective(s) Herpes simplex viruses (HSVs) have widespread and ubiquitous prevalence in the human population and they have received a great deal of attention due to the range of diseases, they caused as a result of an infection. It seems that the fast and reliable diagnostic methods are needed for detecting the herpes simplex virus type 1 (HSV1) antibodies especially in patients with HSV encep...
[ 11 ] - Immunogenicity of a DNA Vaccine Encoding Ag85a-Tb10.4 Antigens from Mycobacterium Tuberculosis
Background: Tuberculosis is a life threatening disease that is partially prevented by BCG vaccine. Development of more effective vaccines is an urgent priority in TB control. Ag85a and Tb10.4 are the members of culture filter protein (CFP) of M. tuberculosis that have high immunogenicity. Objective: To analyze the immunogenicity of Ag85a-Tb10.4 DNA vaccine by enzyme-linked immunosorbent as...
[ 12 ] - Development of Preventive Vaccines for Hepatitis C Virus E1/E2 Protein
Hepatitis C virus (HCV) is responsible for a vast majority of liver failure cases. HCV is a kind of blood disease appraised to chronically infect 3% of the worlds’ population causing significant morbidity and mortality. Therefore, a complete knowledge of humoral responses against HCV, resulting antibodies, and virus-receptor and virus-antibody interactions, are essential to design a vaccine. HC...
[ 13 ] - The Frequency of qnr Genes in Extended-Spectrum β-lactamases and non-ESBLs Klebsiella pneumoniae Species Isolated from Patients in Mashhad, Iran
Background and Objectives: Since the fluoroquinolones are the broad-spectrum antibiotics, they affect both Gram-negative and Gram-positive bacteria. These antibiotics are widely prescribed by physicians. As a result, some bacteria, especially Enterobacteriaceae, have shown a resistance to this family of antibiotics. The current study a...
[ 14 ] - Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran
Background and Objectives: Interferon-gamma is an important cytokine, which facilitates immunity against intracellular pathogens. Several factors, including genetic variations of cytokine-producing genes have been shown to influence the progression and severity of Hepatitis C virus (HCV) infection. Methods: Between January and D...
[ 15 ] - Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in a eukaryot...
[ 16 ] - Designing and construction of a DNA vaccine encoding tb10.4 gene of Mycobacterium tuberculosis
Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...
[ 17 ] - Developing Michigan Cancer Foundation 7 Cells with Stable Expression of E7 Gene of Human Papillomavirus Type 16
Background: Human papillomavirus (HPV) is responsible for the development of cervical neoplasia. Infection with human papillomavirus type 16 (HPV-16) is a major risk factor for the development of cervical cancer. The virus encodes three oncoproteins (E5, E6 and E7), of which, the E7 oncoprotein is the major protein involved in cell immortalization and transformation o...
[ 18 ] - Frequency of Human Papillomavirus Genotypes 6, 11, 16, 18 And 31 in Paraffin-Embedded Tissue Samples of Invasive Breast Carcinoma, North-East of Iran
Background &Objective;: Breast cancer is the most common female malignancy. Detection of DNA of human papillomaviruses (HPVs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. The aim of this study was to evaluate the frequency of HPVs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas. Me...
[ 19 ] - Prevalence of TEM and SHV Genes in Clinical Isolates of Klebsiella Pneumonia From Mashhad, North- East Iran
Background & Objectives:Extended-spectrum-B-lactamase (ESBL)-producing strains of Klebsiella Pneumoniaare an important cause of many serious infections in hospitalized and nonhospitalized patients and delayed treatment of these infections in crease chance of death in patients. This study was performed to determine the prevalence of ESBL-producing K. Pneumonia and to evaluate the frequency of TE...
[ 20 ] - Eight-year Study of Mycobacterium tuberculosis in Mashhad, Northeast of Iran
Background & Objectives: Tuberculosis is one of the greatest health problems in Iran. The distribution of the disease is not equal in all parts of the country. The aim of this study was to evaluate the frequency of positive results for Mycobacterium tuberculosis in samples referred to an academic hospital in an 8 year period. Materials and Methods: The samples from different wards of ...
[ 21 ] - Construction a DNA Vaccine Containing Human Papillomavirus Type 16 Early Genes as a Potential Vaccine for Cervical Cancer Prevention and Therapy
Background and Objectives: Some of the human papillomaviruses (HPVs) can infect genital tracts and are sometimes associated with anogenital tract cancers. HPVs induced cervical cancers through the expression of E6 and E7 genes by inactivating the tumor suppressor proteins. In this study, E6 and E7 genes were chosen in order to construct an expression vector which is able to express ...
[ 22 ] - Hepatitis B Virus Infection and Oral Lichen Planus: A Report from Northeast of Iran
Introduction: Lichen planus is a chronic inflammatory disease in oral mucosa and skin. Recently, reports have demonstrated a possible relationship between lichen planus and liver diseases. During the past decade, there has been a hypothesis regarding viral etiological agents that have been found to be in association with hepatotrophic viruses known as Hepatitis B and C with LP. This research wa...
[ 23 ] - طراحی و ساخت کلونینگ وکتور حاوی قطعه فیوژنی دو ژن hspX و tb 10.4 مایکوباکتریوم توبرکلوزیس
هدف: سل از مهم‌ترین بیماریهای عفونی و از شایعترین علل مرگ و میر در دنیا خصوصاً در کشورهای در حال توسعه میباشد که توسط مایکوباکتریوم توبرکلوزیس ایجاد میشود. طراحی و ساخت واکسن های جدید بر علیه مایکوباکتریوم توبرکلوزیس تنها راه موثر در پیشگیری و کنترل آن میباشد. هدف از این مطالعه طراحی و ساخت کلونینگ وکتور با استفاده از فیوژن ادغام دو ژن hspX و tb10.4 مایکوباکتریوم توبرکلوزیس میباشد. مواد ...
[ 24 ] - پاسخ‌های ایمنی دخیل در عفونت مایکوباکتریوم توبرکلوزیس
زمینه و هدف: مایکوباکتریوم توبرکلوزیس ((M. Tuberculosis، عامل ایجادکننده بیماری سل میباشد. تقریباً یک‌سوم جمعیت جهان با این باکتری آلوده هستند و باوجود دسترسی به واکسن و دارو، عامل مهم مرگ ومیر در انسانها، به‌خصوص در کشورهای در حال توسعه است. مطالعات اپیدمیولوژیک نشان میدهد تنها 30-10% افرادی‌که با باسیل سل برخورد دارند به این باکتری آلوده می‌شوند و در 90% از افراد آلوده و بیشتر، پیشرفت به سم...
[ 25 ] - P 66: A Review of Novel Biomarkers Involved in the Neuroinflammation Caused by Human T Lymphotropic Virus-1
HTLV-1 is the causative agent for a neurologic disease named HTLV-I- associated myelopathy/tropical spastic paraparesis (HAM/TSP). Paraparesis of the lower limbs which appears gradually is the most common clinical feature of this disease. It has been shown that the indirect involvement of the nervous system by lymphocytes is more probable than the direct attack of the virus to the neurons. HTLV...
[ 26 ] - غنی سازی و جداسازی باکتریوفاژهای لیتیک علیه ایزوله های پسودوموناس آئروژینوزا مقاوم به آنتی بیوتیک
زمینه و اهداف: یکی از شایع ترین مشکلات در بیمارستان ها ظهور عفونت های مقاوم به عوامل ضد میکروبی است. باکتریوفاژها هیچ فعالیتی علیه سلول های حیوانی و گیاهی ندارند و تنها در باکتری ها قادر به رشد هستند. بنابراین می توانند بدون زیان رساندن به سلول های آلوده به باکتری، به عنوان جایگزین آنتی بیوتیک استفاده شوند. هدف از این مطالعه غنی سازی و جداسازی باکتریوفاژهای لیتیک علیه ایزوله های پسودوموناس آئر...
[ 27 ] - بررسی الگوی مقاومت آنتی‌بیوتیکی انتروکوک‌های جدا شده از نمونههای بیمارستان قائم مشهد
Background & Aim: Enterococci, gram-positive cocci, are important human pathogens in both community and hospital-acquired infections. Also, they are intrinsically resistant to many commonly used antimicrobial agents. The purpose of this study was to investigate antibiotic resistance profiles of Enterococci isolated from referred patients in Qaem teaching hospital. &n...
[ 28 ] - Comparison of PEG Interferon Loaded and non-Loaded Iron Oxide Nanoparticles on Hepatitis C Virus Replication in Cell Culture System
Background and Aims: Iron oxide nanoparticles are among the most effective tools which can replace current medical techniques for diagnosis and treatment of various diseases. Hepatitis C infection is one of the main health problems in the world, affecting around 3% of the world's population. This infection can develop into liver cirrhosis and liver cancer over the time in 80% of patients. In t...
[ 29 ] - The Antiviral Effects of Curcumin Nanomicelles on the Attachment and Entry of Hepatitis C Virus
Background and Aims: Hepatitis C virus (HCV) is a member of the Flaviviridae family, which causes approximately 500,000 deaths annually. HCV infection treatment is often associated with significant adverse effects. Curcumin is an active ingredient of turmeric which has therapeutic anti-inflammatory effects in many diseases including infectious ones. Although curcumin is not soluble in water, if...
[ 30 ] - Heterologous Expression, Purification, and Characterization of the HspX, Ppe44, and EsxV Proteins of Mycobacterium tuberculosis
Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...
[ 31 ] - CTLA-4 Gene Haplotypes and the Risk of Chronic Hepatitis C Infection; a Case Control Study
Background: The prevalence of hepatitis C virus (HCV) infection is increasing worldwide. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) may play a role in the intensity of the disease. The aim of this study was to evaluate the association between genetic variants of the CTLA-4 and HCV infection. Methods: Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was ...
[ 32 ] - Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
Background: With one-third of the world’s population infected, tuberculosis (TB) is one of the most common infectious diseases and a major public health problem, especially in developing countries. The efficacy of the BCG vaccine for controlling the disease in adults is poor. The development of an effective TB vaccine is a global objective. An effective tuberculosis vaccine should s...
[ 33 ] - Design and Construction of a Cloning Vector Containing the hspX Gene of Mycobacterium tuberculosis
Background: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing the Mycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX). Metho...
[ 34 ] - Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. Methods: ...
[ 35 ] - Designing and Construction of a Cloning Vector Encoding mtb32C and mpt51 Fragments of Mycobacterium Tuberculosis as a DNA Vaccine Candidate
Background & objective: Tuberculosis (TB) remains a major cause of death around the world. Bacillus Calmette Guérin (BCG) is the only vaccine used in TB prevention that has a protective effect in children, but its effectiveness declines in adults. Design and development of new vaccines is the most effective way against TB. The aim of this study was to design and construc...
[ 36 ] - Investigation of the rpoB Mutations Causing Rifampin Resistance by Rapid Screening in Mycobacterium Tuberculosis in North-East of Iran
Background and Objectives: The incidence of rifampin-resistant strains of Mycobacterium tuberculosis has attracted more attention than the tuberculosis infection due to laborious treatment and control. Recognizing the Mycobacterium tuberculosis genotypes involving in drug resistance via multiplex PCR, a simple and rapid genotyping method, is an emergency for better tre...
[ 37 ] - Application of bacterial shuttle vectors in designing new vaccines against infectious diseases: brief report
Background: Today, several vaccines have been developed to prevent infectious diseases. The older first-generation vaccines may have many problems. In this regard, genetic engineering plays an important role using tools such as shuttle vectors to develop recombinant DNA vaccines that usually include plasmid constructed so that can propagate in two different host species. The present study revie...
[ 38 ] - ب‌ث‌ژ تنها واکسن موجود جهت مقابله با بیماری سل: مقاله مروری
Background: Despite advances in the vaccinology and chemotherapy in the past century, tuberculosis is still responsible for two million deaths every year. Emergence of multi-drug resistant strain and coinfection of TB-HIV make it a serious concern. Treatment and control of tuberculosis is a great health burden in every community. Active tuberculosis in children has very severe consequences espe...
[ 39 ] - تعیین فراوانی ژن های VIM 2˓3˓9˓11˓16و VIM all تولیدکننده آنزیم متالو بتالاکتاماز در باکتری پسودوموناس آئروژینوزا
مقدمه꞉مقاومت آنتی بیوتیکی همواره به عنوان یک مشکل جدی برای سلامت انسان مطرح بوده است و بیماران زیادی را در بیمارستان های سراسر جهان تحت تاثیر قرار داده است. پسودوموناس آئروژینوزا یک پاتوژن گرم منفی و یکی از عوامل شایع عفونت های بیمارستانی است. آنزیم های متالو بتالاکتاماز، اصلی ترین مکانیسم مقاومت به آنتی بیوتیک های بتالاکتام در این باکتری می باشد. ژن های تولیدکننده این آنزیم بیشتر بر روی پلاسمی...
[ 40 ] - Application of McCoy Cell Line for Propagation of Herpes Simplex Virus Type 1
Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) are members of the Herpesviridae family. About 40% to 80% of the world populations are infected with HSV and its prevalence is high in Iran. The high prevalence of this virus in the community and the ability of the virus in causing fatal diseases among immunocompromised patients, have encouraged studies to be performed on HSV and suitable cell ...
[ 41 ] - A novel formulation of Mtb72F DNA vaccine for immunization against tuberculosis
Objective(s): Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogen, causes 1.5 million deaths globally. Bacilli Calmette-Guérin (BCG) is commonly administered to protect people against M. tuberculosis infection; however, there are some obstacles with this first-generation vaccine. DNA vaccines, the third generation vaccines, can induce cellular immun...
[ 42 ] - A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses
Objective(s): Infection with tuberculosis (TB) is regarded as a major health issue. Due to the emergence of antibiotic resistance during TB treatment, prevention via vaccination is one of the most effective ways of controlling the infection. DNA vaccines are developed at a greater pace due to their ability in generating a long-lasting immune response, higher safety com...
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