Fatemi N

[ 1 ] - P-65: Effective Parameters on the Bovine Follicle Stimulating Hormone Expression in The Pichia Pastoris System

Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...

[ 2 ] - P-66: Optimization of Human Luteinizing Hormone Expression in CHO Cells Culture by Stepwise Reduction in Serum Concentration

Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

[ 3 ] - P-76: Stepwise Reduction of Fetal Bovine Serum Levels in Chinese Hamster OvaryCells -Expressing Human Chorionic Gonadotrophin- Culture

Background: The demand for producing recombinant therapeutic proteins by mammalian cell lines, such as Chinese hamster ovary (CHO) cells, continues to grow. Significant achievements in process optimization including development of cell culture strategies for largescale and cost-effective production have been made. Fetal bovine serum (FBS) is an often essential growth supplement and yet most cos...

[ 4 ] - P-77: Optimization of Ovine FSH Gene Expression in The Pichiapastoris System byRegulating The Culture Conditions

Background: Ovine follicle stimulation hormone (OFSH) is a pituitary glycoprotein and belongs to the family of glycoprotein hormones. This hormone plays a key role in the function of the reproductive system: it is essential for sertoli cell function and spermatogenesis in testis and it stimulates the growth of ovulatory follicles in females. Ovine FSH hormone is a heterodimeric hormone consisti...

[ 5 ] - P-132: The Effect of Different Fetal Bovine Serum Levels on Recombinant Chinese Hamster Ovary Cells, Expressing HumanFollicle Stimulating Hormone

Background: Follicle stimulating hormone (FSH) is one of the most common and effective gonadotropin hormones used in infertility treatment. Production of FSH as a drug is affordable as long as it is done with minimal cost. FBS is the most expensive component in medium culture furthermore; it’s being controversial to cause unnecessary suffering for the unborn calf for collection their serum. In ...

[ 6 ] - P-152: Expression of Recombinant Human Luteinizing Hormone (hLH) in CHO Cells

Background: Human luteinizing hormone (hLH) stimulates steroid biosynthesis of the ovary, triggers ovulation and prepares androgen production of testicular Leydig cells. LH belongs to the family of glycoprotein hormones that are heterodimers consisting of a common α-subunit and specific β-subunit. hLH is necessary for clinical and infertility treatment. Recombinant DNA technology provides a use...

[ 7 ] - P-205: Production of Recombinant Fish FSH Hormone in Pichia Pastoris

Background: Follicle-stimulating hormone (FSH) belongs to the family of glycoprotein hormones that composing alpha and beta subunits with non-covalently bonds. This hormone involve in regulation of the reproductive processes such as gamete generation and follicular growth. Injection of the hormone in most of fish species increases 17 beta-estradiol production by ovarian tissue and also stimulat...

[ 8 ] - P-199: Effect of Oxidative Stress on Sperm Quality and Mmu-miR-34a Expression in Testis of Mature Mouse

Background: Male infertility is responsible for approximately 50% of infertility in the world. Reactive oxygen species (ROS) is one of the causative agents of infertility in males which effects on sperm quality and function. In this study, the effects of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) were investigated on sperm quality, testis tissue and miRNAs expression. Mater...

[ 9 ] - P-234: Expression of Human Chorionic Gonadotropin (hCG) Hormone Using Chinese Hamster Ovary Cells

Background: Human chorionic gonadotropin (hCG) is a member of glycoprotein hormones family consist of two different non-covalently heterodimeric chains: alpha and beta subunits with 92 and 145 amino acids respectively. This hormone plays an important role in human reproduction and physiology especially for maintenance of the corpus luteum during the first months of pregnancy Materials and Metho...

[ 10 ] - P-87: Effect of Oxidative Stress on Sperm quality and Testis Histopathology in Mature Male Mice

Background: Infertility is a major clinical and worldwide reproductive problem that affects approximately 15% of young couples. The male factors are responsible for periphery 50 percent of these cases. Recently, the generation of oxidative stress, also described as an imbalance between the production of Reactive oxygen species (ROS) and the body’s antioxidant defence mechanisms, in the male rep...