Nasr Esfahani MH

[ 1 ] - I-8: A Preliminary Report on Production of Organ Deficient Goats at Royan Institute of Biotechnology

Shortage of transplantable organs available for people with endstage diseases has become goal of scientific project worldwide to find new approaches for providing the needed organs. These projects have mainly focused on complementation of pig embryo genetically manipulated or designed to produce an organ deficient embryo. Considering the fact that pig is not the common domestic animal in Middle...

[ 2 ] - I-17: Failed Fertilization: from Molecular,Diagnostic and Clinical Perspectives

Low and/or total failed fertilization following ICSI is a disappointing effect that some couples may face during their treatment which may have severe social and economic consequences for these infertile couples. This phenomenon has been mainly attributed to failed oocyte activation post ICSI. Researchers have regarded this failure due to the absence or deficiency of sperm associated to oocyte ...

[ 3 ] - P-11: Evaluation of Heat Shock Protein A2 in Male Rats before and after Varicocele Induction

Background: Varicocele is a major cause of male infertility, as it may impair spermatogenesis through several distinct pathophysiological mechanisms including hypoxia, renal-adrenal reflux, hormonal dysfunction, autoimmunity, oxidative stress and hyperthermia. HSP70s are members of the heat shock protein (HSP) family, which are considered intracellular chaperones. Their expressions can increase...

[ 4 ] - P-12: Effect of Centrifugation on Levels of Stress Oxidative of Human Spermatozoa

Background: Cryopreservation has adverse effect on human spermatozoa via exposure to physical and chemical stress that may lead to alter in reactive oxygen species (ROS) levels. During sperm preparation or cryopreservation, centrifugation as a physical stress may effect on sperm quality probably with stress oxidative production and membrane damage. Therefore the aim of this study was to evaluat...

[ 5 ] - P-90: Effect of Phosphodiesterase Type3 Inhibitor, Cilostamide, on The Developmental Competence of Ovine OocytesIsolated by Glucose 6-Phosphate Dehydrogenase Activity

Background: The developmental competence of oocytes matured in vitro (IVM) is yet far below than in vivo counterparts. Recent studies suggest that the asynchrony between nuclear/cytoplasmic maturation and the initial low/heterogeneous quality of oocytes are the most important factors affecting IVM success. We investigated whether selection of growing oocytes (based on their glucose 6- phosphate...

[ 6 ] - P-100: Improved Efficiency of Somatic Cell Nuclear Transfer in Sheep by Persecution of Recipient Oocytes with Brilliant Cresyl Blue Staining

Background: The most important factor affecting the efficiency of somatic cell nuclear transfer (SCNT) is the initial quality of the oocyte. Since the abattoir-derived oocytes are heterogeneous in quality, selection of cohort fully grown/competent oocytes is necessary for proper development of SCNT embryos. Materials and Methods: Glucose-6-phosphate dehydrogenase (G6PDH) is actively expressed i...

[ 7 ] - P-108: Stepwise Pattern of Reactive Oxygen Species during In Vitro Development of Cloned Goat Embryos

Background: Although testicular tissue cryopreservation could be advantageous for fertility preservation in cancerous children, the possibility of apoptosis incidence should not be ignored. Our aim was evaluation of the apoptosis incidence in mouse testicular tissue after vitrification and short-term culture. Materials and Methods: Testes of 7days old NMRI male mice were isolated, divided and d...