Shirin Kouhpayeh
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81746-73461 Iran
[ 1 ] - The Increase in Protein and Plasmid Yields of E. coli with Optimized Concentration of Ampicillin as Selection Marker
Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...
[ 2 ] - Real-Time PCR: an Appropriate Approach to Confi rm ssDNA Generation from PCR Product in SELEX Process
Background: Aptamers are single stranded DNA (ssDNA) or RNA molecules. The potential of aptamers for binding to the different targets has made them be widely used as the preferred diagnostic and therapeutic tools. DNA aptamers present several advantages over the RNA oligonucleotides due to their higher stability, easier selection, and production. Selection of DNA aptamers which...
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