Shabani, A.

[ 1 ] - Isolation and long-term culture of neural stem cells from Acipenser persicus (Borodin, 1897)

In the present study, an in vitro brain cell culture was developed from neural cells of Persian sturgeon (Acipenser persicus). The tissue samples collected from the anterior, middle and posterior regions of the brain were cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was refreshed every 3 days. The cells became confluen...