Azim Akbarzadeh

[ 1 ] - Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp. (H14)

In this study, mutant forms of Bacillus thuringiensis spp. israelensis (H14) were produced. These mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. Protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. The protoplasts were induced to fuse to each other i...