توکلی, احمد

[ 1 ] - مروری بر انواع روش‌های Real-time PCR و بهینه‌سازی آن

     روش Real-time PCR در سال‌های اخیر به ­عنوان روشی انتخابی جهت تشخیص بسیاری از بیماری‌های عفونی در آزمایشگاه­ ها مورد استفاده قرار گرفته است. با استفاده از این فن آوری، واکنش زنجیره پلیمراز توسط مولکول‌های گزارش ­گر فلورسنت انجام و از این طریق، تولید فرآورده‌های تکثیری طی هر چرخه واکنش PCR گزارش می‌شود. بدین ترتیب مجموعه‌ای از خصوصیات از جمله؛ حساسیت و اختصاصیت بالا، داده‌های قابل اعتماد و خ...

[ 2 ] - Frequency of human Parvovirus B19 among patients with respiratory infection in Iran

Background: Human parvovirus B19 was known as one of the possible cause of mild respiratory tract diseases in previous studies. However, there are some reports of acute obstructive respiratory disease and severe pneumonia. The purpose of current study was to assess the prevalence and clinical features of parvovirus B19 in respiratory infection.    Methods: This study was conducted on...

[ 3 ] - No molecular evidence of Hepatitis E infection among patients with HIV in Iran

[ 4 ] - No Evidence for Human Papillomavirus in Patients with HIV in Iran

Background: Human Papillomaviruses (HPVs) have anestablished role in the development of cervical cancer. However, the presence of underlying conditions such as HIV/AIDS is necessary for this to occur. This studyaimed to evaluate the prevalence of HPV DNA in plasma samples from HIV-positive patients in Tehran, Iran. Methods: Plasma specimens from 95 patients diagnosed with HIV infection from Te...

[ 5 ] - Xenotropic Murine leukemia virus-related virus (XMRV) in Iranian HIV Patients

Background: Despite of isolation of Xenotropic murine leukaemia virus-related virus (XMRV) from the patients acquired prostate cancer in 2006 and patients with chronic fatigue syndrome in 2009, there have been controversial findings about its potential role in human diseases and frequencies in different population groups. In the present study, we aimed to determine the frequency of XMRV genome ...

[ 6 ] - مروری جامع بر عفونت ویروس زیکا

Zika virus is a mosquito-borne flavivirus that is becoming a new global threat. Currently, serologic (ELISA) and molecular (RT-PCR) testing make the diagnosis of Zika virus infection. There is no specific drug available for Zika virus infection, and treatment is supportive based on reducing symptoms of disease. Protection against mosquito bites is the most effective way to prevent the infection...

[ 7 ] - Molecular Detection of Human Cytomegalovirus in Semen of infertile Men in Tehran

Background and Aims: Human cytomegalovirus (HCMV) can induce latency and evade the immune system. The latent virus can reactive later in older ages and due to immunosuppressive conditions. Sexually transmitted diseases and viruses can influence the male reproductive system, and members of the Herpesviridae family are one of these important viruses. Regarding the importance of herpesviruses, spe...

[ 8 ] - A Brief Review on the Advancement of the Molecular and Serological Diagnosis of SARS-CoV-2

The novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has introduced as causative agent for coronavirus disease 2019 (COVID-19) since the 2019 December. Human coronaviruses are classified in Nidovirales order and Coronavirdiae family. This family includes four genera. The SARS-CoV-2 is a member of Betacoronavirus genera and Sarbecovirus linage (linage B). There is ...

[ 9 ] - In Vitro Assessment of The Effects of Althaea Officinalis Root Extract on Rotavirus Multiplication

Background and Aims: Medicinal plants possess a variety of beneficial characteristics without causing substantial adverse effects. Antimicrobial activity is one of the qualities, among many others, that have been identified. Althaea officinalis is an annual plant belonging to the Malvaceae family with therapeutic qualities in both the leaves and the roots. Specifically, the effects of Althaea o...

[ 10 ] - Antiviral Activity of Arnebia Euchroma and Amniotic Membrane against HSV-1, Rotavirus, and Influenza Virus

Background and Aims: Currently, there are antiviral chemicals used to treat viral infections accompanied by limitations such as high levels of toxicity and adverse effects in humans, the emergence of drug-resistant viral strains, low numbers, and limited diversity. Therefore, it is necessary to evaluate new photochemical to obtain new therapeutic methods. The present study was conducted to eval...

[ 11 ] - Prevalence of Human Cytomegalovirus Infection in Iranian Prostate Cancer Patients

Background and Aim: Human cytomegalovirus (HCMV) has the potential for oncogenicity and has been associated with many malignancies, including prostate cancer. This study aimed to investigate the genomic prevalence of HCMV in prostate cancer tumors. Materials and Methods: In this case-control study, 31 patients with prostate cancer were enrolled along with 31 patients with benign prostate cance...