رسولی , مهدی

[ 1 ] - پرفیوژن و جداسازی هپاتوسیت از کبد رات

Background and Purpose: Perfused rat liver and isolated hepatocytes are useful to study specific metabolism of this organ. Traditionally, mechanical, chemical and enzymatic methods have been used to isolate hepatocytes, and it appears that a mixed method is the preferred choice. Materials and Methods: The rat liver perfused in situe in three subsequent steps, 10 minutes with Krebs-Ringer s...