یاری, فاطمه
دانشیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
[ 1 ] - همسانه‌سازی و بیان ژن نوترکیب CD40Lانسانی در رده سلولی HEK293
چکیده سابقه و هدف CD40L ، پروتئینی غشایی و یکی از اعضای خانواده TNF است که نقش مهمی در انتقال پیام سلولی در ایمنی ذاتی و تطبیقی دارد. از آن جایی‌که این پروتئین نقش خود را از طریق تجمع گیرنده در سطح سلول هدف ایفا می‌کند، به نظر می‌رسد فرم مالتی‌‌مری آن بتواند اثر قوی‌تری نسبت به فرم طبیعی ترایمری ایجاد کند. بر این اساس در این مطالعه کلونینگ و بیان فرم کایمری و دودکامری CD40L محلول، از طریق پر...
[ 2 ] - تاثیر مهارگر کاسپاز 3 بر بقای پلاکت کنسانتره در طول زمان ذخیره
چکید ه سابقه و هدف زمان نگهداری فرآورده کنسانتره پلاکتی به 5-3 روز محدود می‌شود. یکی ازعلل آن، پدیده آسیب‌های زمان ذخیره( storage lesion )، آسیب به سلول‌های پلاکتی و نهایتاً آپوپتوز است که در طول مدت نگهداری در این سلول‌ها القا می‌شود. مطالعه‌ها نشان داده است که آپوپتوز در بعضی سلول‌های بدون هسته و از جمله پلاکت‌ها نیز رخ می‌دهد. در این مطالعه، به کارگیری مهارگرآنزیم کاسپاز3 در زمان ذخیره ...
[ 3 ] - تغییرات میزان کموکاین PF4 و P-Selectin در پلاکت کنسانتره در طول زمان نگهداری در دو محیط مختلف پلاسما و کمپوسول
چکید ه سابقه و هدف کیفیت فرآورده پلاکت کنسانتره، تحت تاثیر روش تهیه و شرایط ذخیره آن می‌باشد. حذف پلاسما از محیط ذخیره پلاکتی می‌تواند یک روش مؤثر در افزایش سلامت و یا افزایش بقای آن در طول زمان ذخیره پلاکت به شمار آید. در این راستا، مقایسه دو محیط پلاسما و کمپوسول جهت نگهداری پلاکت کنسانتره مورد مطالعه و ارزیابی قرار گرفت. مواد و روش‌ها در یک مطالعه تجربی، کیسه کنسانتره پلاکتی از پای...
[ 4 ] - میکروپارتیکل‌های مشتق از پلاکت در دو محیط مختلف پلاسما و کمپوسول
میکروپارتیکل‌های مشتق از پلاکت در دو محیط مختلف پلاسما و کمپوسول شیما آزادپور1، فاطمه یاری2، شهرام وائلی3 چکید ه سابقه و هدف پلاکت‌های فعال شده در پاسخ به محرک‌های خاص، میکروپارتیکل‌ها را در بدن و یا با گذشت زمان، در طول ذخیره در کیسه پلاکتی، آزاد می‌نمایند. هدف از این مطالعه، مقایسه تاثیر دو محیط ذخیره متفاوت شامل پلاسما و محلول افزودنی(کمپوسول) بر تولید میکروپارتیکل‌های مشتق ا...
[ 5 ] - القای انفجار تنفسی در سلول‌های منوسیت انسانی پس از مواجهه با میکروپارتیکل‌های پلاکتی
سابقه و هدف: پلاکت‌های فعال شده در کنسانتره پلاکتی، وزیکول‌های غشایی آزاد می‌کنند که میکروپارتیکل‌های پلاکتی نامیده شده و می‌توانند به‌طور خود به‌خود و طی نگهداری پلاکت‌ها ایجاد شوند. مطالعات نشان می‌دهند که میکروپارتیکل‌های پلاکتی می‌توانند با سلول‌های منوسیت تشکیل توده سلولی بنمایند لذا در صدد برآمدیم تا توانایی این میکرووزیکول‌ها را در فعال کردن سلول‌های منوسیت بررسی کنیم. مواد و روش‌ها: ک...
[ 6 ] - تاثیر پلاکت در فرآورده کنستانتره پلاکتی بر فعال‌سازی سلول‌های منوسیت خون محیطی انسان
Introduction: Monocytes can interact with platelets due to their surface molecules such as P-selectin glycoprotein ligand-1 (PSGL-1), and form monocyte-platelet complex. In the present study, the effects of platelets interaction of platelet concentrates (PCs) and peripheral blood monocytes were investigated in vitro as a model to predict the probable interactions of these cells and consequently...
[ 7 ] - بررسی تاثیر میکروذرات مشتق از پلاکت بر تولید آنتی بادی IgG از لنفوسیت های Bخون محیطی انسان
Background and purpose: Platelets communicate with different immune cells and can activate B-lymphocytes and induce the production of antibodies from these cells. Platelet microparticles (MPs) originate from platelets and express the surface markers of platelets. This study aimed at investigating the ability of these microvesicles on production of antibodies from B-lymphocytes. Materials and...
[ 8 ] - تاثیر گلیکوزیلاسیون برعملکرد و فاگوسیتوز پلاکت ها در طول نگهداری فرآورده پلاکت کنسانتره در سرما
Background and purpose: Cold storage results in clustering of gpIbα molecules on platelets. Consequently, the lectin domains of αMβ2 receptors on the liver macrophages recognize these molecules and ingest the platelets. Probably coverage of sugar molecules of gpIbα using galactose could prevent the recognition and ingestion of platelets by macrophages. The purpose of this study therby was to...
[ 9 ] - Induction of apoptosis in human tumor cell lines by platelets
Introduction: It has been reported that platelets can eradicate tumor cells in vitro, although the mechanism of this effect has not been determined. The effect of platelets on the induction of apoptosis in tumor cells is largely unknown. Materials and methods: To investigate this effect, two human hematologic cell lines, K562 and Daudi, were independently faced with unstimulated and thromb...
[ 10 ] - Isolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...
[ 11 ] - Mesenchymal stem cells as a reference cell for HLA-typing
Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high e...
[ 12 ] - Microvesicles preparation from mesenchymal stem cells
Background: Extracellular vesicles are particles ranged from 30 nm to 5µm and subcategorized into three groups; exosomes, microvesicles and apoptotic bodies, each of which have different biological impact. Lack of a standard method for the detection and isolation of MVs has led to a challenging issue that is a worth considering. In this study, we isolated MVs from the conditioned medium o...
[ 13 ] - Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
Background and Aims: Ficolins are proteins that bind to carbohydrates, act as opsonins and play an important role in innate immunity. Polymorphism in ficolin-3 gene (FCN3) can lead to complement deficiency and increase the risk of some disorders such as diabetes. The aim of this study was to investigate the frequency of FCN3+1637delC as a single nucleotide polymorphism in this gene in healthy a...
[ 14 ] - The source of HLA molecules on platelets: Does platelets adsorb soluble HLA molecules from their environment?
Background: The origin and function of human leukocyte antigen (HLA) class I molecules on platelets are still highly arguable. Given the differences in the results of the previous studies in this regard, the lack of research in recent years, and the clinical importance of HLA class I molecules, the absorption capacity of platelets for soluble HLA class I molecules was studied in this investigat...
[ 15 ] - The role of platelet microparticles in the production of antibodies from B lymphocytes against HLA-DR antigen in vitro
Background: Platelets can activate B cells and stimulate them for the production of antibodies. Since platelet microparticles (PMPs) originate from platelets, they may have the same virtue. In the present study, the effect of PMPs was investigated on the production of human leukocyte antigen (HLA)-specific antibody from B cells in vitro. Materials and Methods: In this experimental study, HLA-D...
[ 16 ] - The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate
Background: Storage of platelet concentrates (PCs) at room temperature (20-24°C) limits its storage time to 5 days due to the destructive effects of platelet storage lesion (PSL) and bacterial contamination. Although prolonged storage of platelets (PLTs) at 4°C reduces the likelihood of bacterial contamination and PSL levels, it is accompanied by an increase in the clearance rate and changes in...
[ 17 ] - Platelet Refractoriness and how it is formed
Abstract Background and Objectives Transfusion of platelet has become one of the main treatments for thrombocytopenic patients to reduce the severity and frequency of bleeding consequences. Failure to attain the desired level of platelets in a patient following platelet transfusion is defined as platelet refractoriness. The purpose of present paper is to review and study the mechanisms involve...
[ 18 ] - Identification, Prevention and management of platelet refractoriness
Abstract Background and Objectives Platelet refractoriness can be caused by immunological and non-immunological factors. In non-immunological cases, the source which is usually a disease, should be eliminated. In immunological cases antibodies produced against foreign platelet antigens play a significant role. The aim of this review was to investigate the strategies involved in identifying ...
[ 19 ] - Frequency of rare HLA-A allelic groups in Gilak, Lor, Kurdish and Arab ethnic groups of Iran
Abstract Background and Objectives Histocompatibility antigens (HLAs) have high genetic diversity and their frequency can be diverse in different ethnicities. Therefore, the determination of these antigens in different ethnicities can provide data used in the expansion of centers for the registration of stem cell donors. Materials and Methods In this descriptive study, donor DNA was extracted ...
[ 20 ] - Evaluation of effective factors in the optimization of immunization to achieve antibodies against RhD antigen in culture medium
Abstract Background and Objectives Antibody against RhD antigen is important in blood transfusion and clinical medicine. Therefore, in this study, the effective factors in the optimization of in vitro immunization to achieve immunized lymphocytes that produce antibodies were evaluated to obtain antibodies against RhD antigen in the culture medium. Materials and Methods In an experimental study...
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