Ahmad Gharehbaghian

[ 1 ] - Variations in Intraplatelet Phospho-VASP Expression Due to Pre-analytical Sample Preparations, Illustration of a Quality Control Issue in Platelet Pharmacology

Intraplatelet phospho-VASP analysis is a commonly used approach for monitoring of anti-platelet therapy; however, testing of intraplatelet phospho-VASP expression needs a high level of experience and proficiency. The ability to recognize how the pre-analytical variations can change the results would be helpful for the interpretation of data from intraplatelet P-VASP analysis. The aim of this st...

[ 2 ] - Variations in Intraplatelet Phospho-VASP Expression Due to Pre-analytical Sample Preparations, Illustration of a Quality Control Issue in Platelet Pharmacology

Intraplatelet phospho-VASP analysis is a commonly used approach for monitoring of anti-platelet therapy; however, testing of intraplatelet phospho-VASP expression needs a high level of experience and proficiency. The ability to recognize how the pre-analytical variations can change the results would be helpful for the interpretation of data from intraplatelet P-VASP analysis. The aim of this st...

[ 3 ] - تاثیر عصاره متانولی بخش‌های هوایی گیاه درمنه خزری(Artemisia annua) بر تکثیر و مرگ سلول‌های سرطانی لوسمی لنفوبلاستیک حاد رده‌های Nalm-6 و Reh

چکیده سابقه و هدف لوسمی لنفوبلاستیک حاد(ALL)، شایع‌ترین بدخیمی در کودکان محسوب می‌شود. با توجه به اثرات جانبی شیمی درمانی، امروزه استفاده از ترکیبات طبیعی برای درمان بدخیمی‌ها مورد توجه بسیار قرار گرفته است. اثر مهاری آرتمیسیا آنوا(درمنه خزری) بر روی چندین رده سلولی سرطانی گزارش شده است. هدف از این مطالعه، بررسی اثر سمیت این عصاره بر رده‌های سلولی لوسمی لنفوبلاستیک حاد بود. مواد و روش‌ها در ای...

[ 4 ] - تاثیر عصاره بخش‌های هوایی گیاه اُرس(Juniperus excelsa) بر تکثیر و مرگ سلول‌های سرطانی لوسمی لنفوبلاستیک حاد رده‌های Nalm-6 و Reh

چکیده سابقه و هدف لوسمی لنفوبلاستیک حاد(ALL)، شایع‌ترین بدخیمی در کودکان است. به دلیل اثرات جانبی فراوان داروهای شیمی‌درمانی، استفاده از ترکیبات طبیعی مورد توجه قرار گرفته است. اُرس یک گیاه دارویی است که اثر مهاری عصاره آن بر روی چندین رده سلولی سرطانی گزارش شده است. هدف از این مطالعه، بررسی اثر کشندگی و آپوپتوتیک عصاره بخش‌های هوایی گیاه اُرس بر رده‌های سلولی ALL ( Nalm-6 و Reh) بود. مواد و رو...

[ 5 ] - تأثیر سایتوتوکسیک سه گونه گیاهی جنس گل گندم (Centaurea) بر رده سلولی لوسمی لنفوبلاستیک حاد (Nalm-6)

مقدمه: امروزه گیاهان دارویی به سبب محتوای زیست فعال و سمیت انسانی محدود به عنوان منبع ایده‌آل برای پیشگیری یا درمان سرطان مورد توجه قرار گرفته‌اند. گیاهان جنس Centaurea که تأثیر سایتوتوکسیک آنها بر چندین رده سلولی سرطانی در مطالعات پیشین نشان داده شده است، دسته‌ای از این گیاهان محسوب می‌شوند. هدف: در این مطالعه، تأثیر سایتوتوکسیک سه گونه Centaurea (C. albonitens، C. pseudoscabiosa و C. salicifo...

[ 6 ] - Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line

Objective(s): microRNAs are small non-coding molecules that regulate gene expression in various biological processes. T-cell acute lymphoblastic leukemia (T-ALL) is a malignancy accompanied with genetic aberrations and accounts for 20% of children’s and adult’s ALL. Notch signaling pathway dysregulation occurs in 60% of T-ALL cases. In the present study, we aimed to de...

[ 7 ] - CDK Blockade Using AT7519 Suppresses Acute Myeloid Leukemia Cell Survival through the Inhibition of Autophagy and Intensifies the Anti-leukemic Effect of Arsenic Trioxide

The strong storyline behind the critical role of cyclin-dependent kinase (CDK) inhibitor proteinsin natural defense against malignant transformation not only represents a heroic perspective forthese proteins, but also provides a bright future for the application of small molecule inhibitorsof CDKs in the novel cancer treatment strategies. The results of the present study revea...

[ 8 ] - Proteasome Inhibition by Carfilzomib Induced Apotosis and Autophagy in a T-cell Acute Lymphoblastic Leukemia Cell Line

T-cell acute lymphoblastic leukemia is an aggressive hematologic malignancy which is usuallyassociated with unfavorable prognosis particularly in patients with refractory/relapsed disease.Therefore, development of novel therapeutic strategies is highly required for improving theoutcome of these patients. Although there are several studies evaluating the efficacy of proteasome<...

[ 9 ] - CDK Blockade Using AT7519 Suppresses Acute Myeloid Leukemia Cell Survival through the Inhibition of Autophagy and Intensifies the Anti-leukemic Effect of Arsenic Trioxide

The strong storyline behind the critical role of cyclin-dependent kinase (CDK) inhibitor proteinsin natural defense against malignant transformation not only represents a heroic perspective forthese proteins, but also provides a bright future for the application of small molecule inhibitorsof CDKs in the novel cancer treatment strategies. The results of the present study revea...

[ 10 ] - Proteasome Inhibition by Carfilzomib Induced Apotosis and Autophagy in a T-cell Acute Lymphoblastic Leukemia Cell Line

T-cell acute lymphoblastic leukemia is an aggressive hematologic malignancy which is usuallyassociated with unfavorable prognosis particularly in patients with refractory/relapsed disease.Therefore, development of novel therapeutic strategies is highly required for improving theoutcome of these patients. Although there are several studies evaluating the efficacy of proteasome<...

[ 11 ] - The prevalence of undesired blood group antibodies in thalassemia patients

Abstract Background and Objectives Blood transfusion related alloimmunization is common among patients receiving regular blood transfusion, especially thalassemia patients. In this study we aimed to find the prevalence of alloimmunization among patients of thalassemia. Materials and Methods In this desciptional study, we screened 305 thalassemia patients (53.8% female and 46.2% male, age ...

[ 12 ] - The Effect of Gaillardin on Proliferation and Apoptosis of Acute Lymphoblastic Leukemia Cell Line (Nalm-6)

Background: Due to increased cancer chemotherapy resistance, the use of natural compounds in cancer treatment has been considered in recent years. Inula-Oculus-Christi, is one of the high importance plants in the field of traditional medicine. It has been shown that extract of this plant has cytotoxic effects on breast cancer cells. Objective: Therefore, the aim of this study was to evaluate t...

[ 13 ] - The apoptotic potential of ergolide to induce apoptosis in molt4 cell lines

Background: Cancer is a multi-faceted diseases caused cell proliferation in an out of control manner due to accumulation of defects and mutation in their DNA and with an impendence to invade or spread to parts of the body. During last decades new compounds with natural roots have emerged as a new paradigm for effective anti-cancer treatment. Objective: The aim of this study is to evaluate the p...

[ 14 ] - Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6)

Abstract Background and Objectives Acute lymphoblastic leukemia is a hematological malignancy of lymphoid lineage. Side effects of chemotherapy drugs is a major reason for the tendency to use Herbal medicines. Previous studies on Juniperus excelsa (J.excelsa) have shown anti-cancer properties on some cancer cell lines. This study aimed to investigate the effect of three fractions of J.excelsa ...