Seyed Nasser Ostad

[ 1 ] - Anti-invasion Effects of Cannabinoids Agonist and Antagonist on Human Breast Cancer Stem Cells

Studies show that cancer cell invasion or metastasis is the primary cause of death inmalignancies including breast cancer. The existence of cancer stem cells (CSCs) in breast cancermay account for tumor initiation, progression, and metastasis. Recent studies have reporteddifferent effects of cannabinoids on cancer cells via CB1 and CB2 cannabinoid receptors. In thepresent study, the effects of ...

[ 2 ] - The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat

Lithium (Li), a glycogen synthase kinase-3β (GSK-3β) inhibitor, has used to attenuate thecannabinoid-induced dependence/withdrawal signs, but molecular mechanisms related to this areunclear. Recent studies indicate the involvement of upstream extracellular signal kinase1/2 (ERK1/2)and downstream GSK-3β pathways in the development of cannabinoid-induced dependence. Thisis mediated through cannab...

[ 3 ] - Neuroprotective Effect of Total and Sequential Extract of Scrophularia striata Boiss. in Rat Cerebellar Granule Neurons Following Glutamate- Induced Neurotoxicity: An In-vitro Study

Neuroprotective effect of the extract from aerial parts of Scrophularia striata Boiss(Scrophulariaceae) was investigated against glutamate-induced neurotoxicity on cultured rat pupsCerebellar Granule Neurons (CGNs). CGNs from 8 days old Sprague-Dawley rat were preparedand cultured. The experiments were performed after 8 days in culture. The plant was collected fromthe northeastern part (Ruin re...

[ 4 ] - A Comprehensive Review of Punica granatum (Pomegranate) Properties in Toxicological, Pharmacological, Cellular and Molecular Biology Researches

Punica granatum (Pg), commonly known as pomegranate (Pg), is a member of the monogeneric family, Punicaceae, and is mainly found in Iran which is considered to be its primary centre of origin. Pg and its chemical components possess various pharmacological and toxicological properties including antioxidant, anti-inflammatory (by inhibiting pro-inflammatory cytokines), anti-cancer and anti-angiog...

[ 5 ] - Investigation of Cytotoxic Activity in Four Stachys Species from Iran

The aerial parts of Stachys laxa Boiss. and Buhse.from Siah-bishe in Mazandaran province, Stachys trinervis Aitch. and Hemsl. from Karaj in Alborz province, Stachys subaphylla Rech. F. and Stachys turcomanica Trautv. from Golestan province have been collected in May 2008. Total extracts were obtained through MeOH/H2O (80/20) and then partitioned between CHCl3, EtOAc and MeOH. These fractions...

[ 6 ] - The Time Course of JNK and P38 Activation in Cerebellar Granule Neurons following Glucose Deprivation and BDNF Treatment

Low glucose condition induces neuronal cell-death via intracellular mechanisms including mitogen-activated protein kinases (MAPK) signaling pathways. It has been shown that low glucose medium decreases neuronal survival in cerebellar granule neurons (CGNs). In this study, we have examined the activation of JNK, p38kinase and ERK1/2 pathways in low glucose medium in CGNs. The CGNs were prepared ...

[ 7 ] - Anti-invasion Effects of Cannabinoids Agonist and Antagonist on Human Breast Cancer Stem Cells

Studies show that cancer cell invasion or metastasis is the primary cause of death inmalignancies including breast cancer. The existence of cancer stem cells (CSCs) in breast cancermay account for tumor initiation, progression, and metastasis. Recent studies have reporteddifferent effects of cannabinoids on cancer cells via CB1 and CB2 cannabinoid receptors. In thepresent study, the effects of ...

[ 8 ] - The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat

Lithium (Li), a glycogen synthase kinase-3β (GSK-3β) inhibitor, has used to attenuate thecannabinoid-induced dependence/withdrawal signs, but molecular mechanisms related to this areunclear. Recent studies indicate the involvement of upstream extracellular signal kinase1/2 (ERK1/2)and downstream GSK-3β pathways in the development of cannabinoid-induced dependence. Thisis mediated through cannab...

[ 9 ] - Neuroprotective Effect of Total and Sequential Extract of Scrophularia striata Boiss. in Rat Cerebellar Granule Neurons Following Glutamate- Induced Neurotoxicity: An In-vitro Study

Neuroprotective effect of the extract from aerial parts of Scrophularia striata Boiss(Scrophulariaceae) was investigated against glutamate-induced neurotoxicity on cultured rat pupsCerebellar Granule Neurons (CGNs). CGNs from 8 days old Sprague-Dawley rat were preparedand cultured. The experiments were performed after 8 days in culture. The plant was collected fromthe northeastern part (Ruin re...

[ 10 ] - A Comprehensive Review of Punica granatum (Pomegranate) Properties in Toxicological, Pharmacological, Cellular and Molecular Biology Researches

Punica granatum (Pg), commonly known as pomegranate (Pg), is a member of the monogeneric family, Punicaceae, and is mainly found in Iran which is considered to be its primary centre of origin. Pg and its chemical components possess various pharmacological and toxicological properties including antioxidant, anti-inflammatory (by inhibiting pro-inflammatory cytokines), anti-cancer and anti-angiog...

[ 11 ] - Investigation of Cytotoxic Activity in Four Stachys Species from Iran

The aerial parts of Stachys laxa Boiss. and Buhse.from Siah-bishe in Mazandaran province, Stachys trinervis Aitch. and Hemsl. from Karaj in Alborz province, Stachys subaphylla Rech. F. and Stachys turcomanica Trautv. from Golestan province have been collected in May 2008. Total extracts were obtained through MeOH/H2O (80/20) and then partitioned between CHCl3, EtOAc and MeOH. These fractions...

[ 12 ] - The Time Course of JNK and P38 Activation in Cerebellar Granule Neurons following Glucose Deprivation and BDNF Treatment

Low glucose condition induces neuronal cell-death via intracellular mechanisms including mitogen-activated protein kinases (MAPK) signaling pathways. It has been shown that low glucose medium decreases neuronal survival in cerebellar granule neurons (CGNs). In this study, we have examined the activation of JNK, p38kinase and ERK1/2 pathways in low glucose medium in CGNs. The CGNs were prepared ...

[ 13 ] - The effect of nimesulide on CoxII expression in central and peripheral immune cells (microglia and macrophage) in a rat model of neuropathic pain

Introduction: Neuropathic pain may be due to a primary insult to the peripheral or central nervous system. In this situation, Hyperalgesia and Allodynia are the results of prostaglandins and cytokines release in the spinal cord. It seems that immune cells play an importat role in the induction and maintenance of chronic pain. Compared to selective CoxII inhibitors, nimesulide, a highly select...

[ 14 ] - N-Terminal Domain of Fragile Histidine Triad Exerts Potent Cytotoxic Effect in HT1080 Cells

Fragile histidine triad (FHIT) serves a critical function as a tumor suppressor that inhibits p53 degradation by mouse double minute 2 (MDM2). The functional domains of FHIT involved in tumor inhibition was interpreted. In-silico screening data were employed to construct truncated forms of FHIT to assess their cytotoxic effects on the HT1080 cell line. Full FHIT expression was confirmed by west...

[ 15 ] - N-Terminal Domain of Fragile Histidine Triad Exerts Potent Cytotoxic Effect in HT1080 Cells

Fragile histidine triad (FHIT) serves a critical function as a tumor suppressor that inhibits p53 degradation by mouse double minute 2 (MDM2). The functional domains of FHIT involved in tumor inhibition was interpreted. In-silico screening data were employed to construct truncated forms of FHIT to assess their cytotoxic effects on the HT1080 cell line. Full FHIT expression was confirmed by west...